Neuronal Activity of the Medulla Oblongata Revealed by Manganese-Enhanced Magnetic Resonance Imaging in a Rat Model of Gastroesophageal Reflux-Related Cough
Neuronal Activity of the Medulla Oblongata Revealed by Manganese-Enhanced Magnetic Resonance Imaging in a Rat Model of Gastroesophageal Reflux-Related Cough
复制标题
锰增强磁共振成像显示胃食管反流性咳嗽大鼠模型中延髓的神经元活动
DOI：
10.33549/physiolres.933791
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发表时间：
2019-01-01
影响因子：
2.1
通讯作者：
Lai, Kefang
中科院分区：
文献类型：
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作者：
Chen, Zhe;Gu, Dachuan;Lai, Kefang
我们研究了胃食管反流相关咳嗽（GERC）期间延髓的神经元活动。通过下食管灌注HCl并用柠檬酸诱导咳嗽建立GERC大鼠模型。 HCl组大鼠接受HCl灌注，未出现柠檬酸引起的咳嗽。盐水对照大鼠改为用盐水灌注并诱导咳嗽。柠檬酸致咳嗽大鼠仅由柠檬酸诱导。空白组大鼠正常喂养。采用免疫组织化学方法观察延髓核中 Fos 的表达。腹腔注射 MnCl2 后，进行锰增强磁共振成像 (MEMRI) 检测 Mn2+ 信号。 HCl灌注和柠檬酸诱导咳嗽导致孤束核（nTS）、迷走神经背运动核（DMV）、旁三叉核（Pa5）和中间网状核（IRt）中Fos表达高于HCl组、盐水对照组、柠檬酸诱导咳嗽组和空白组。与空白组动物相比，模型大鼠的大多数细胞核中也观察到高 Mn2+ 信号。与空白组动物相比，HCl、盐水和柠檬酸诱导的咳嗽组动物的 Mn2+ 信号也较高。研究表明，在HCl灌注和柠檬酸诱导的咳嗽大鼠模型中，延髓神经元处于兴奋状态，nTS、DMV、Pa5和IRt神经元可能参与咳嗽过程和信号整合。
We investigated neuronal activity of the medulla oblongata during gastroesophageal reflux-related cough (GERC). A rat model of GERC was generated by perfusing HCl into lower esophagus and inducing cough with citric acid. The HCl group rat was received HCl perfusion without citric acid-induced cough. The saline control rat was perfused with saline instead and cough was induced. Citric acid-induced cough rat was only induced by citric acid. Blank group rats were fed normally. Fos expressions were observed in medulla oblongata nuclei using immunohistochemistry. Manganese-enhanced magnetic resonance imaging (MEMRI) was performed to detect the Mn2+ signal following intraperitoneal injection of MnCl2. HCl perfusion and citric acid-induced cough caused Fos expressions in the nucleus of solitary tract (nTS), dorsal motor nucleus of the vagus (DMV), paratrigeminal nucleus (Pa5), and intermediate reticular nucleus (IRt), which was higher than HCl group, saline control group, citric acid-induced cough group, and blank group. A high Mn2+ signal was also observed in most of these nuclei in model rats, compared with blank group animals. The Mn2+ signal was also higher in the HCl, saline and citric acid-induced cough group animals, compared with blank group animals. The study showed medulla oblongata neurons were excited in a HCl perfusion and citric acid-induced cough rat model, and nTS, DMV, Pa5 and IRt neurons maybe involved in the cough process and signal integrate.