Diversity of the ribulose bisphosphate carboxylase/oxygenase form I gene (rbcL) in natural phytoplankton communities
Diversity of the ribulose bisphosphate carboxylase/oxygenase form I gene (rbcL) in natural phytoplankton communities
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DOI：
10.1128/aem.63.9.3600-3606.1997
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发表时间：
1997-09
影响因子：
4.4
通讯作者：
S. Pichard;Lisa Campbell;John H. Paul
中科院分区：
文献类型：
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作者：
S. Pichard;Lisa Campbell;John H. Paul
世界海洋中的浮游植物通过将二氧化碳转化为有机碳，在全球碳循环和食物网中发挥着不可或缺的作用。它们通过Calvin循环酶核酮糖-1，5-二磷酸羧基酶/加氧酶(Rubisco)的作用来完成这一任务。在这里，我们通过mRNA或DNA扩增和480~483bp的内部片段测序，研究了公海自然浮游植物群落和海洋自养微微型浮游植物的代表性克隆中IrbcL基因的系统发育多样性。从墨西哥湾天然浮游植物群落的核酸中恢复了五个基因序列。对两株原氯球菌和一株聚球藻(WH8007)的rbcL基因进行了序列测定。将序列与rbcL基因数据库进行比对，并进行相邻连接和简约分析。来自天然浮游植物群落的5个序列几乎涵盖了特征的I型rbcL基因的全部多样性，其中一些序列与聚球藻和原氯球菌(I型)和裸藻(I型)等分离株关系密切，而其他序列则是根深蒂固的。出人意料的是，深海真光带中含有一种具有转录活性的rbcL基因，该基因与最近鉴定的一种锰氧化细菌的rbcL基因密切相关，这表明这种化学自养生物可能有助于海洋真光带中固碳生物的多样性。
The phytoplankton of the world's oceans play an integral part in global carbon cycling and food webs by conversion of carbon dioxide into organic carbon. They accomplish this task through the action of the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Here we have investigated the phylogenetic diversity in the form I rbcL locus in natural phytoplankton communities of the open ocean and representative clones of marine autotrophic picoplankton by mRNA or DNA amplification and sequencing of a 480 to 483 bp internal fragment of this gene. Five gene sequences were recovered from nucleic acids of natural phytoplankton communities of the Gulf of Mexico. The rbcL genes of two Prochlorococcus isolates and one Synechococcus strain (WH8007) were also sequenced. Sequences were aligned with the database of rbcL genes and subjected to both neighbor-joining and parsimony analyses. The five sequences from the natural phytoplankton community spanned nearly the entire diversity of characterized form I rbcL genes, with some sequences closely related to isolates such as Synechococcus and Prochlorococcus (forms IA and I) and prymnesiophyte algae (form ID), while other sequences were deeply rooted. Unexpectedly, the deep euphotic zone contained an organism that possesses a transcriptionally active rbcL gene closely related to that of a recently characterized manganese-oxidizing bacterium, suggesting that such chemoautotrophs may contribute to the diversity of carbon-fixing organisms in the marine euphotic zone.