Gene expression analysis of the CD4+ T-cell clones derived from gingival tissues of periodontitis patients
Gene expression analysis of the CD4+ T-cell clones derived from gingival tissues of periodontitis patients
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DOI：
10.1111/j.1399-302x.2005.00241.x
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发表时间：
2005-12-01
影响因子：
--
通讯作者：
Yamazaki, K
中科院分区：
文献类型：
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作者：
Ito, H;Honda, T;Yamazaki, K
T细胞在牙周炎病变中的作用是复杂的，目前尚未完全阐明。在这里，我们从牙周炎患者的牙龈组织中建立了T细胞克隆，并检测了它们的基因表达。用固相化抗CD3抗体和IL-2分别从牙龈组织和外周血中建立了57个和101个T细胞克隆。牙周T细胞克隆来自三名患者，外周血T细胞克隆来自其中两名患者和另一名其牙周T细胞克隆未建立的患者。另外19例牙周炎患者的牙周组织也被采集。逆转录-聚合酶链式反应检测与调节功能和组织破坏相关的细胞因子和分子的表达。所有牙周T细胞克隆均表达转化生长因子-β1、CTLA-4和CD25的mRNA，外周血T细胞克隆均表达干扰素-γ和转化生长因子-β1的mRNAs。大部分(但不是全部)牙龈组织和外周血的T细胞克隆分别表达干扰素-γ、CD25和CTLA-4的mRNA。FOXP3可能是小鼠CD4(+)CD25(+)调节性T细胞的主要基因，表达FOXP3的T细胞克隆和牙龈组织的频率非常高(牙龈T细胞克隆、外周血T细胞克隆和牙龈组织分别为97%、93%和100%)。而表达IL-4的T细胞克隆的频率在牙龈T细胞克隆中较低(70%比87%)，而表达IL-10和IL-17的T细胞克隆的频率高于外周血T细胞克隆(IL-10的75%比62%，IL-17的51%比11%)。与牙周组织标本相比，牙龈T细胞克隆的表达谱相似，除了IL-4的表达外，牙龈组织中阳性样本的频率较低(70%对11%)。这些结果表明，单个T细胞可以同时表达Th1和Th2细胞因子以及调节细胞因子的mRNA。
The function of T cells infiltrating periodontitis lesions is complex and has not been fully elucidated. Here, we established T-cell clones from the gingival tissues of periodontitis patients and examined their gene expression. A total of 57 and 101 T-cell clones were established by means of immobilized anti-CD3 antibody and IL-2 from gingival tissues and peripheral blood, respectively. The gingival T-cell clones were derived from three patients, and the peripheral blood T-cell clones from two of these patients and a further patient whose gingival T-cell clones were not established. Gingival tissues were also obtained from a further 19 periodontitis patients. The expression of cytokines and molecules related to both regulatory function and tissue destruction were examined by means of reverse-transcription polymerase chain reaction. All the gingival T-cell clones expressed mRNA for TGF-beta 1, CTLA-4, and CD25, and all the T-cell clones from peripheral blood expressed IFN-gamma and TGF-beta 1 mRNAs. Most but not all the T-cell clones from gingival tissues and peripheral blood expressed mRNA for IFN-gamma and, CD25 and CTLA-4, respectively. The frequency of T-cell clones and gingival tissues expressing FOXP3, a possible master gene for mouse CD4(+)CD25(+) regulatory T cells, was very high (97%, 93%, and 100% for gingival T-cell clones, peripheral blood T-cell clones, and gingival tissues, respectively). Whereas the frequency of IL-4-expressing T-cell clones was lower for gingival T-cell clones (70% vs. 87%), the frequency of the gingival T-cell clones expressing IL-10 and IL-17 was higher than peripheral blood T-cell clones (75% vs. 62% for IL-10, 51% vs. 11% for IL-17). A similar expression profile was observed for gingival T-cell clones compared with gingival tissue samples with the exception of IL-4 expression, where the frequency of positive samples was lower in the gingival tissues (70% vs. 11%). These results suggest that the individual T cells infiltrating gingival lesions can express mRNA for both Th1 and Th2 cytokines as well as regulatory cytokines simultaneously.