THE EXTRACTION AND ELEMENTAL ANALYSIS OF IRON PLAQUE FROM THE SURFACE OF RICE ROOTS
THE EXTRACTION AND ELEMENTAL ANALYSIS OF IRON PLAQUE FROM THE SURFACE OF RICE ROOTS
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发表时间：
2007
影响因子：
4.3
通讯作者：
W. Guodong
中科院分区：
文献类型：
--
作者：
W. Guodong
The objective of this paper was to identify a non-sulfur containing extractant to extract iron plaque on the surface of rice roots.Compared the iron plaque extraction efficiency of six treatment combinations consisting of three reducing reagents(hydroxylamine,ascorbic acid,1/2hydroxylamine+1/2ascorbic acid)and two buffer solution systems(bicarbonate and acetic acid).Sodium citrate was used as a complexing agent.The elemental content in the extractions was analyzed using inductively coupled plasma(ICP)emission spectroscopy.The results showed that the amounts of Fe and Mn extracted from iron plaque on rice root surfaces were affected by the type of reducing reagent and the buffer pH.The ascorbic-citrate-acetic solution(ACA)had the highest extraction efficiency among the six extraction treatments.Specifically,57307 mg Fe·kg-1 were extracted with ACA which was equivalent to 99% of the Fe extracted with DCB.Similarly,1689 mg Mn·kg-1 were extracted with ACA,which was 109% of the Mn extracted with DCB.The Fe,Mn,P and S in the ACA solution was simultaneously determined by ICP,and the corresponding coefficient of variations were lower than in DCB extracts.These results indicate that the ACA-ICP combination is an effective method for the extraction and elemental analysis of iron plaque from the root surface of rice.
