Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing
Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing
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耐热马克斯克鲁维酵母和酿酒酵母菌株代表了通过综合生物加工从菊芋生产生物乙醇的潜力
DOI：
10.1007/s00253-012-4240-8
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发表时间：
2012-09-01
影响因子：
5
通讯作者：
Li, Fu-Li
中科院分区：
文献类型：
--
作者：
Hu, Nan;Yuan, Bo;Li, Fu-Li
利用耐热菊粉的酵母菌通过联合生物处理(CBP)从菊芋块茎中生产乙醇是可取的。为了获得这类菌株，对21株自然产生的酵母菌和65株以前分离的酿酒酵母菌株进行了菊粉利用、胞外菊粉酶活力测定和40℃下菊粉和菊芋块茎粉酒精发酵的研究，其中马氏克鲁维酵母PT-1(CGMCC AS2.4515)和酿酒酵母JZ1C(CGMCC AS2.3878)的胞外菊粉酶活力和乙醇产量最高。在40℃下发酵菊芋粉(200g L(-1))时，马氏克氏杆菌PT-1和酿酒酵母JZ1C的最高乙醇浓度分别为73.6g L(-1)和65.2g L(-1)，分别相当于理论酒精得率的90.0%和79.7%。在30~40℃的温度范围内，温度对两个菌株的乙醇产量没有显著影响。本研究显示了马氏克氏杆菌PT-1和酿酒酵母JZ1C在菊芋耐热性和菊粉类低聚糖利用方面的独特优势。结果表明，马氏克氏杆菌和酿酒酵母在高温CBP法生产菊芋块茎乙醇方面具有较大的潜力。
Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 °C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L(-1)) at 40 °C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L(-1), which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 °C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP.