A QTL for flowering time in Arabidopsis reveals a novel allele of CRY2
A QTL for flowering time in Arabidopsis reveals a novel allele of CRY2
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DOI：
10.1038/ng767
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发表时间：
2001-12-01
期刊：
影响因子：
30.8
通讯作者：
Koornneef, M
中科院分区：
文献类型：
--
作者：
El-Assal, SED;Alonso-Blanco, C;Koornneef, M
开花时间的变化在许多植物物种的自然群体中被发现。潜在的遗传变异，主要是一个数量的性质，被认为是反映了适应不同的环境有助于繁殖成功。对拟南芥开花时间的自然变异的分析已经确定了几个数量性状基因座（QTL）（1），这些基因座尚未在分子水平上表征。决定开花时间的一个主要环境因素是光周期或日长，光周期的长度，随着地理纬度的不同而在一年中发生变化（2）。我们将EDI基因座鉴定为QTL，其部分解释了两种拟南芥品种之间开花对光周期反应的差异：源自北方欧洲的实验室品系兰茨贝格erecta（Ler）和采集于热带佛得角群岛的Cvi（3）。EDI QTL的定位克隆显示它是一个新的等位基因，编码蓝光感光器隐花色素-2，先前已显示在长日照（LD）光周期中促进开花（4）。我们表明，独特的EDI开花表型的结果，从一个单一的氨基酸取代，减少了光诱导的下调，在短的光周期下生长的植物中，导致早花。
Variation of flowering time is found in the natural populations of many plant species. The underlying genetic variation, mostly of a quantitative nature, is presumed to reflect adaptations to different environments contributing to reproductive success. Analysis of natural variation for flowering time in Arabidopsis thaliana has identified several quantitative trait loci (QTL)(1), which have yet to be characterized at the molecular level. A major environmental factor that determines flowering time is photoperiod or day length, the length of the light period, which changes across the year differently with geographical latitude(2). We identified the EDI locus as a QTL partly accounting for the difference in flowering response to the photoperiod between two Arabidopsis accessions: the laboratory strain Landsberg erecta (Ler), originating in Northern Europe, and Cvi, collected in the tropical Cape Verde Islands(3). Positional cloning of the EDI QTL showed it to be a novel allele of CRY2, encoding the blue-light photoreceptor cryptochrome-2 that has previously been shown to promote flowering in long-day (LD) photoperiods(4). We show that the unique EDI flowering phenotype results from a single amino-acid substitution that reduces the light-induced downregulation of CRY2 in plants grown under Short photoperiods, leading to early flowering.