A PROMOTERLESS PERIOD GENE MEDIATES BEHAVIORAL RHYTHMICITY AND CYCLICAL PER EXPRESSION IN A RESTRICTED SUBSET OF THE DROSOPHILA NERVOUS-SYSTEM
A PROMOTERLESS PERIOD GENE MEDIATES BEHAVIORAL RHYTHMICITY AND CYCLICAL PER EXPRESSION IN A RESTRICTED SUBSET OF THE DROSOPHILA NERVOUS-SYSTEM
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DOI：
10.1016/0896-6273(94)90212-7
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发表时间：
1994-03-01
期刊：
影响因子：
16.2
通讯作者：
HALL, JC
中科院分区：
文献类型：
--
作者：
FRISCH, B;HARDIN, PE;HALL, JC
转基因苍蝇携带的7.2 kb的DNA片段的周期（每）基因进行了分析的存在下，昼夜运动活动的节奏和波动的前编码的mRNA和蛋白质。该基因组片段的5'端位于第一内含子内，其位于编码区之前。这种无启动子的片段可以拯救昼夜行为节律，并介导PER在CNS和PNS内的野生型PER细胞亚群中的空间表达。在一条行为节律线中，PER蛋白仅在“每侧神经元中发现。“在节律性转基因中，每mRNA和蛋白质水平经历昼夜节律循环，正如之前对野生型所描述的那样。在热休克启动子的控制下，在携带相同的7.2kb per DNA片段的果蝇脑细胞中，PER的循环证实了PER的分子循环和行为节律性是因果关系的假设。
Transgenic flies carrying a 7.2 kb piece of DNA from the period (per) gene were analyzed for the presence of circadian locomotor activity rhythms and fluctuations of per-encoded mRNA and protein. The 5' end of this genomic fragment is within the first intron, which precedes the coding region. This promotorless fragment could rescue circadian behavioral rhythms and mediate spatial expression of PER in a subset of wild-type per cells within the CNS and PNS. In one behaviorally rhythmic line, PER protein was found in only ''per lateral neurons.'' In the rhythmic transgenics, per mRNA and protein levels undergo circadian cycling, as previously described for wild type. Cycling of PER in brain cells of flies carrying the same 7.2 kb piece of per DNA under the control of a heat shock promotor corroborated the hypothesis that per's molecular cyclings and behavioral rhythmicity are causally related.