Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae.
Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae.
复制标题
Ady3p 将酿酒酵母中纺锤体极体功能与孢子壁合成联系起来。
DOI：
10.1093/genetics/160.4.1439
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Neiman,AaronM
中科院分区：
文献类型：
--
作者：
Nickas,MarkE;Neiman,AaronM
酵母的孢子形成需要原孢子膜和孢子壁的重新合成。Ady 3 p是Mpc 70 p/Spo 21 p和Don 1 p的相互作用伴侣，Mpc 70 p/Spo 21 p是前孢子膜形成所必需的纺锤体极体外斑的减数分裂特异组分，Don 1 p在减数分裂II期间在前孢子膜前缘形成环状结构。我们在这里报告说，在双杂交试验中，Ady 3 p与SPB外部和中央斑块的其他成分相互作用。缺乏ADY 3的细胞表现出孢子形成效率的降低，并且大多数形成的3 Δ/ady 3 Δ子囊含有少于4个孢子。ady 3 Δ/ady 3 Δ细胞的产孢缺陷是由于不能合成孢子壁聚合物。Ady 3 p在减数分裂II纺锤体周围形成环状结构，这些纺锤体与Don 1 p形成的纺锤体共定位，并且在ady 3 Δ/ady 3 Δ细胞中，在减数分裂II期间不存在Don 1 p环。在mpc 70 Δ/mpc 70 Δ细胞中，Ady 3 p在减数分裂II期间保持与SPB结合。我们的研究结果表明，Ady 3 p介导的装配Don 1 p的含结构的前孢子膜的前缘通过与组件的SPB的相互作用，这种结构是参与孢子壁的形成。
Spore formation inSaccharomyces cerevisiaerequires thede novosynthesis of prospore membranes and spore walls. Ady3p has been identified as an interaction partner for Mpc70p/Spo21p, a meiosis-specific component of the outer plaque of the spindle pole body (SPB) that is required for prospore membrane formation, and for Don1p, which forms a ring-like structure at the leading edge of the prospore membrane during meiosis II.ADY3expression has been shown to be induced in midsporulation. We report here that Ady3p interacts with additional components of the outer and central plaques of the SPB in the two-hybrid assay. Cells that lackADY3display a decrease in sporulation efficiency, and mostady3Δ/ady3Δ asci that do form contain fewer than four spores. The sporulation defect inady3Δ/ady3Δ cells is due to a failure to synthesize spore wall polymers. Ady3p forms ring-like structures around meiosis II spindles that colocalize with those formed by Don1p, and Don1p rings are absent during meiosis II inady3Δ/ady3Δ cells. Inmpc70Δ/mpc70Δ cells, Ady3p remains associated with SPBs during meiosis II. Our results suggest that Ady3p mediates assembly of the Don1p-containing structure at the leading edge of the prospore membrane via interaction with components of the SPB and that this structure is involved in spore wall formation.