Repetitive A epitope (type 3 chain A) defined by blood group A1-specific monoclonal antibody TH-1: chemical basis of qualitative A1 and A2 distinction.
Repetitive A epitope (type 3 chain A) defined by blood group A1-specific monoclonal antibody TH-1: chemical basis of qualitative A1 and A2 distinction.
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DOI：
10.1073/pnas.82.4.1199
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发表时间：
1985-02
影响因子：
11.1
通讯作者：
H. Clausen;S. Levery;E. Nudelman;S. Tsuchiya;S. Hakomori
中科院分区：
文献类型：
--
作者：
H. Clausen;S. Levery;E. Nudelman;S. Tsuchiya;S. Hakomori
建立了能与A1血型特异反应，但不与A2或O红细胞反应的IgG2a单抗TH-1。在血凝和抗体吸附试验中，该抗体仅与A1红细胞反应；即使经胰酶或唾液酸酶处理后，它也不与A2红细胞反应。这种抗体在TLC免疫染色中检测到A1红细胞的一系列糖脂，而A2红细胞几乎没有或非常弱的条带。它不与类型1或类型2的链A或与Globo-A反应。最简单的反应组分是由乙酰化化合物的HPTLC从先前指定的AB组分中分离出来的。反应组分的结构通过1H核磁共振波谱、甲基化分析和酶降解进行了表征，如下所示：(公式：见正文)。该结构本质上是附着在2型链上的重复的A表位，因此被称为3型链A。该决定簇可以在扩展和/或分支结构上携带，但在糖蛋白中检测不到。这种结构是A1红细胞的特征，在A2红细胞中只有微量存在。前体H(Fucα1-2GalNAcα1-3[Fucα1-3[Fucα1-2]GalBβ1-4GlcNAcβ1-R；3型链H)在A2红细胞中的含量高于A1红细胞，但在O和B红细胞中均不存在。A1转移酶明显可以将α-GalNAc转移到3型链H，而A2转移酶可能没有这种能力。
The IgG2a monoclonal antibody TH-1, which reacts specifically with blood group A1 but with neither A2 nor O erythrocytes, has been established. The antibody reacted only with A1 erythrocytes in hemagglutination and antibody absorption assays; it did not react with A2 erythrocytes, even after trypsin or sialidase treatment. This antibody detected, on TLC immunostaining, a series of glycolipids from A1 erythrocytes but virtually none or very weak bands from A2 erythrocytes. It did not react with type 1 or type 2 chain A, or with globo-A. The simplest reactive component was isolated from a previously assigned Ab fraction by HPTLC of acetylated compounds. The structure of the reactive component was characterized by 1H NMR spectroscopy, methylation analysis, and enzymatic degradation, as shown below: (Formula: see text). The structure is essentially a repetitive A epitope attached to type 2 chain and is hereby called type 3 chain A. The determinant can be carried on extended and/or branched structures, but it was not detectable in glycoproteins. The structure was characteristic of A1 erythrocytes and present in only trace amounts in A2 erythrocytes. The precursor H (Fuc alpha 1----2Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----4GlcNAc beta 1----R; type 3 chain H) was present in greater quantity in A2 erythrocytes than in A1 erythrocytes, but it was absent in both O and B erythrocytes. The A1 transferase apparently can transfer alpha-GalNAc to type 3 chain H, while the A2 transferase may not have this ability.