Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana
Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana
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DOI：
10.1007/s00709-010-0119-7
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发表时间：
2010-06-01
期刊：
影响因子：
2.9
通讯作者：
Abe, Tomoko
中科院分区：
文献类型：
--
作者：
Fujiwara, Makoto T.;Hashimoto, Haruki;Abe, Tomoko
尽管花粉质体在细胞质遗传中发挥着至关重要的作用，但其行为和繁殖仍然难以捉摸。在这里，我们展示了转基因拟南芥系花粉粒和生长管中质体的实时图像，这些转基因拟南芥系以营养细胞特异性方式表达基质定位的 FtsZ1-绿色荧光蛋白融合蛋白。成熟花粉中的营养细胞含有圆形至椭圆形质体的形态异质群体，而晚期发育（成熟）花粉中的营养细胞包含可能具有一两个收缩位点的质体。此外，花粉管中的质体表现出显着的管状、基质（基质填充的小管）延伸以及沿着管生长方向的来回运动。在成熟花粉粒中很少观察到涉及 FtsZ1 环的质体分裂。
The behaviour and multiplication of pollen plastids have remained elusive despite their crucial involvement in cytoplasmic inheritance. Here, we present live images of plastids in pollen grains and growing tubes from transgenic Arabidopsis thaliana lines expressing stroma-localised FtsZ1-green-fluorescent protein fusion in a vegetative cell-specific manner. Vegetative cells in mature pollen contained a morphologically heterogeneous population of round to ellipsoidal plastids, whilst those in late-developing (maturing) pollen included plastids that could have one or two constriction sites. Furthermore, plastids in pollen tubes exhibited remarkable tubulation, stromule (stroma-filled tubule) extension, and back-and-forth movement along the direction of tube growth. Plastid division, which involves the FtsZ1 ring, was rarely observed in mature pollen grains.