Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint.
Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint.
复制标题
DOI：
10.1038/s41598-017-03629-5
复制
发表时间：
2017-06-13
影响因子：
4.6
通讯作者：
Clark I
中科院分区：
文献类型：
--
作者：
Davidson R;Gardner S;Jupp O;Bullough A;Butters S;Watts L;Donell S;Traka M;Saha S;Mithen R;Peffers M;Clegg P;Bao Y;Cassidy A;Clark I
骨关节炎是残疾的主要原因，目前没有药物治疗可以预防这种疾病或减缓其进展。饮食建议或补充剂显然是一个有吸引力的选择，因为它具有低毒性和易于在人群水平上实施。我们以前已经证明，萝卜硫素，一种膳食异硫氰酸酯，来源于西兰花中发现的硫代葡萄糖酸盐前体，可以在体外和体内骨关节炎模型中预防细胞中的软骨破坏。作为本研究的下一阶段，我们招募了40例接受全膝关节置换术的膝关节骨关节炎患者进入原理验证试验。患者在手术前14天随机接受低或高硫代葡萄糖酸盐饮食。我们在高硫代葡萄糖甙组的滑液中检测到ITCs，但在低硫代葡萄糖甙组中未检测到ITCs。这反映在ITC的增加，特别是在血浆中的萝卜硫素。滑液的蛋白质组学分析显示，125个差异表达的蛋白质组之间有显着不同的配置文件。这种饮食的功能后果现在将在临床试验中进行测试。
Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level. We have previously demonstrated that sulforaphane, a dietary isothiocyanate derived from its glucosinolate precursor which is found in broccoli, can prevent cartilage destruction in cells, in in vitro and in vivo models of osteoarthritis. As the next phase of this research, we enrolled 40 patients with knee osteoarthritis undergoing total knee replacement into a proof-of-principle trial. Patients were randomised to either a low or high glucosinolate diet for 14 days prior to surgery. We detected ITCs in the synovial fluid of the high glucosinolate group, but not the low glucosinolate group. This was mirrored by an increase in ITCs and specifically sulforaphane in the plasma. Proteomic analysis of synovial fluid showed significantly distinct profiles between groups with 125 differentially expressed proteins. The functional consequence of this diet will now be tested in a clinical trial.