Ultrastructural immunocytochemical characterization of the thyrotroph in rat and human pituitaries.
Ultrastructural immunocytochemical characterization of the thyrotroph in rat and human pituitaries.
复制标题
大鼠和人垂体促甲状腺素的超微结构免疫细胞化学特征。
DOI：
10.1177/24.11.794404
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
R. B. Tobin
中科院分区：
文献类型：
--
作者：
G. C. Moriarty;R. B. Tobin
用未标记抗体-过氧化物酶-抗过氧化物酶复合物技术和抗大鼠或牛促甲状腺激素（TSH）β抗血清，对正常大鼠和人垂体内TSH β链进行了定位。Araldite包埋的组织，TSH的免疫反应性最好的保存后，用1%戊二醛或苦味酸甲醛固定。在TSH细胞中，免疫细胞化学染色位于颗粒上。然而，在单个颗粒的染色和颗粒群之间存在变化。大鼠TSH细胞呈卵圆形或角状至星状，含有大小为60-175 nm的颗粒。人促甲状腺激素细胞是多面体的，含有分散的颗粒，直径为150-300 nm。在两种植物中，颗粒分布在细胞质的边缘，或在细胞质中散布，并在细胞质突起中有一定的浓度。动情期雌性大鼠TSH细胞内颗粒较多。促甲状腺激素细胞与促肾上腺皮质激素细胞和促黄体激素细胞的颗粒大小和分布，细胞形状的基础上区分。
The beta chain of thyroid stimulating hormone (TSH) was localized in normal rat and human pituitaries with the unlabeled antibody--peroxidase-antiperoxidase complex technique and antisera to rat or bovine TSH beta. Araldite embedded tissues, immunoreactivity of TSH was best preserved after fixation with 1% glutaraldehyde or picric acid formaldehyde. In the TSH cells, the immunocytochemical stain was located on granules. However, there was a variation in staining of individual granules, and among the population of granules. Rat TSH cells were ovoid or angular to stellate, and contained granules ranging in size from 60-175 nm. The human TSH cell was polyhedral, and contained scattered granules of 150-300 nm diameter. In both species, granule distribution was either in 1-2 rows at the periphery, or scattered throughout the cytoplasm with some concentration in cytoplasmic processes. TSH cells in female rats in estrous contained more granules than those of other stages. TSH cells were distinguished from adrenocorticotropic hormone cells and luteinizing hormone cells on the basis of granule size and distribution, and cell shape.