Detection of Mg(2+)-dependent endonuclease activity in myeloid leukemia cell nuclei capable of producing internucleosomal DNA cleavage.
Detection of Mg(2+)-dependent endonuclease activity in myeloid leukemia cell nuclei capable of producing internucleosomal DNA cleavage.
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检测髓系白血病细胞核中能够产生核小体间 DNA 切割的 Mg(2) 依赖性核酸内切酶活性。
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
M. Okuma
中科院分区：
文献类型：
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作者：
H. Kawabata;N. Anzai;H. Masutani;T. Hirama;Y. Yoshida;M. Okuma
我们用自身消化法将自体白血病细胞的染色质切割成寡核小体长度模式，检测了非凋亡髓系白血病细胞核中Mg（2+）依赖性、Ca（2+）非依赖性核酸内切酶活性。在人白血病细胞核的蛋白质提取物中可以成功地回收类似的核酸内切酶活性。提取物一致地引起另一种白血病细胞（KG-1）细胞核作为靶的特征性DNA切割，其酶活性已经失活。我们建议，这种方法是一个有用的工具，参与细胞凋亡的核酸内切酶的研究。
We detected Mg(2+)-dependent, Ca(2+)-independent endonuclease activity in non-apoptotic myeloid leukemia cell nuclei using autodigestion method which cleaved the chromatin of the autologous leukemia cells to an oligonucleosomal length pattern. Similar endonuclease activity could be successfully recovered in the protein extracts of the human leukemia cell nuclei. The extracts consistently elicited characteristic DNA cleavage of another leukemia cell (KG-1) nuclei as the target, the enzyme activity of which had been inactivated. We propose that this method is a useful tool for the study of endonucleases involved in apoptosis.