Regulation of Notch output dynamics via specific E(spl)-HLH factors during bristle patterning in Drosophila
Regulation of Notch output dynamics via specific E(spl)-HLH factors during bristle patterning in Drosophila
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DOI：
10.1038/s41467-019-11477-2
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发表时间：
2019-08-02
影响因子：
16.6
通讯作者：
Schweisguth, Francois
中科院分区：
文献类型：
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作者：
Couturier, Lydie;Mazouni, Khalil;Schweisguth, Francois
成蝇胸上的感觉刚毛的定型排列源于自组织过程，其中抑制性Notch信号既限定了前神经条，又挑出了感觉器官前体细胞（SOPs）。前神经因子和分裂-HLH（E（spl）-HLH）Notch靶增强子之间的动态平衡是图案形成的基础，但这是如何调节的尚不清楚。在这里，我们确定了两类E（spl）-HLH因子，其表达既先于前神经活动又界定前神经活动，并且分别取决于前神经活动和条纹内适当SOP间距所需。这两类是部分冗余的，因为第二类的成员通常会被第一类的成员交叉抑制，可以在功能上弥补它们的缺失。前神经因子对特定E（spl）-HLH基因的调节放大了对Notch的反应，因为SOP正在被选择，有助于背中的模式化动力学，并且可能在其他发育环境中起作用。
The stereotyped arrangement of sensory bristles on the adult fly thorax arises from a self-organized process, in which inhibitory Notch signaling both delimits proneural stripes and singles out sensory organ precursor cells (SOPs). A dynamic balance between proneural factors and Enhancer of split-HLH (E(spl)-HLH) Notch targets underlies patterning, but how this is regulated is unclear. Here, were identify two classes of E(spl)-HLH factors, whose expression both precedes and delimits proneural activity, and is dependent on proneural activity and required for proper SOP spacing within the stripes, respectively. These two classes are partially redundant, since a member of the second class, that is normally cross-repressed by members of the first class, can functionally compensate for their absence. The regulation of specific E(spl)-HLH genes by proneural factors amplifies the response to Notch as SOPs are being selected, contributing to patterning dynamics in the notum, and likely operates in other developmental contexts.