Propanil affects transcriptional and posttranscriptional regulation of IL-2 expression in activated EL-4 cells
Propanil affects transcriptional and posttranscriptional regulation of IL-2 expression in activated EL-4 cells
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DOI：
10.1006/taap.1998.8545
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发表时间：
1999-01-15
影响因子：
3.8
通讯作者：
Barnett, JB
中科院分区：
文献类型：
--
作者：
Zhao, W;Schafer, R;Barnett, JB
酰胺类除草剂敌稗在动物模型中引起许多免疫调节作用。在本研究中，我们研究了敌稗对小鼠淋巴瘤T细胞系EL-4中IL-2表达和产生的影响。当用佛波醇12-肉豆蔻酸酯13-乙酸酯在敌稗存在下刺激细胞的上清液通过酶联免疫吸附测定进行评估时，IL-2水平被20和50 μ M的敌稗剂量依赖性地降低，但在10 μ M时不降低。峰值IL-2信息水平的定量北方印迹分析也显示剂量依赖性降低。然而，信息产生的动力学模式却没有受到影响。为了确定减少的信息产生是否是由于减少的信号传导或信息稳定性，进行了核连续和mRNA稳定性测定。核连续测定确定IL-2基因的转录速率在20 μ M敌稗存在下降低约50%，表明其能够干扰信号转导。IL-2信息稳定性测定也证明了信息稳定性的降低。因此，敌稗似乎通过影响信号转导途径和IL-2信息稳定性来减少IL-2的产生。(C)北京：科学出版社.
The amide-class herbicide, propanil, causes numerous immunomodulary effects in animal models. In the present study, we investigated the effect of propanil on IL-2 expression and production in the murine lymphoma T cell line, EL-4. When supernatants of cells stimulated with phorbol 12-myristate 13-acetate in the presence of propanil were assessed by enzyme-linked immunosorbent assay, IL-2 levels were dose-dependently decreased by 20 and 50 mu M of propanil but not at 10 mu M. Quantitative Northern blot analysis of peak IL-2 message levels also showed a dose-dependent decrease. The kinetic pattern of message production, however, was unaffected. To determine if the reduced message production was due to reduced signaling or message stability, nuclear run-on and mRNA stability assays were performed. Nuclear run-on assays determined that the transcription rate of the IL-2 gene was decreased approximately 50% in the presence of 20 mu M propanil, indicating that it was able to interfere with signal transduction. IL-2 message stability assays also demonstrated a reduction in message stability. Thus, propanil appears to reduce IL-2 production by affecting the signal transduction pathway and IL-2 message stability. (C) 1999 Academic Press.