Localization and Up-Regulation of Cysteinyl Leukotriene-2 Receptor in Human Allergic Nasal Mucosa
Localization and Up-Regulation of Cysteinyl Leukotriene-2 Receptor in Human Allergic Nasal Mucosa
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DOI：
10.2332/allergolint.12-oa-0490
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发表时间：
2013-06-01
影响因子：
6.8
通讯作者：
Himi, Tetsuo
中科院分区：
文献类型：
--
作者：
Shirasaki, Hideaki;Kanaizumi, Etsuko;Himi, Tetsuo
背景：半胱氨酰白三烯（CysLTs）是一种脂质介质，与变应性鼻炎的发病机制有关。使用CysLT的药理学研究表明存在2类受体，即CysLT（1）和CysLT（2）受体。前一类受体对目前用于治疗哮喘和变应性鼻炎的CysLT（1）拮抗剂敏感，我们的研究小组先前已使用免疫组织化学和原位杂交技术对其定位进行了研究。我们通过免疫印迹和免疫组织化学分析研究了CysLT（2）受体在人鼻粘膜中的表达和定位。方法：取16例药物治疗无效的鼻阻塞患者的下鼻甲，行下鼻甲切除术。结果：抗CysLT（2）受体抗体在人鼻甲骨标本的Westernblot上可检测到一条分子量为39kDa的蛋白带，而抗CysLT（2）受体抗体在人鼻甲骨标本的Western blot上可检测到一条分子量为39kDa的蛋白带。鼻变态反应患者CysLT（2）受体的表达水平高于非变应性鼻炎患者。结论：CysLT（2）受体在上呼吸道血管反应中起主要作用。
Background: Cysteinyl leukotrienes (CysLTs) are lipid mediators that have been implicated in the pathogenesis of allergic rhinitis. Pharmacological studies using CysLTs indicate that 2 classes of receptors exist, namely, CysLT(1) and CysLT(2) receptors. The former class of receptors is sensitive to the CysLT(1) antagonists currently used to treat asthma and allergic rhinitis, and its localization has been previously examined by our group using immunohistochemistry and in situ hybridization techniques. We investigated the expression and localization of the CysLT(2) receptor in human nasal mucosa by western blot and immunohistochemical analyses.Methods: Human turbinates were obtained after turbinectomy from 16 patients with nasal obstruction refractory to medical therapy. To identify the cells expressing the CysLT(2) receptor, double immunostaining was performed by using anti-CysLT(2) receptor antibody and anti-CD31 (endothelial cell) antibody or anti-smooth muscle actin antibody.Results: A 39 kDa band was detected on the western blots of human turbinates samples by using the anti-CysLT(2) receptor antibody. The expression level of the CysLT(2) receptor in patients with nasal allergy was higher than that in patients with non-allergic rhinitis. The immunohistochemical study also showed an intense immunoreactivity for CysLT(2) receptor in both vascular endothelial cells and vascular smooth muscles.Conclusions: The results indicated that the CysLT(2) receptor plays a primary role in the vascular responses in the upper respiratory tract.