Three steps of neural stem cells development in gerbil dentate gyrus after transient ischemia
Three steps of neural stem cells development in gerbil dentate gyrus after transient ischemia
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DOI：
10.1097/00004647-200204000-00005
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发表时间：
2002-04-01
影响因子：
6.3
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Iwai, M;Sato, K;Abe, K
神经发生的阶段可分为三个步骤：增殖、迁移和分化。为了阐明缺血后这三个步骤之间的详细关系，作者评价了短暂性全脑缺血5分钟后成年沙鼠齿状回（DG）中的三个步骤，分别使用溴脱氧尿苷（BrdU）、高聚唾液酸化神经细胞粘附分子（PSA-NCAM）、神经元核抗原（NeuN）和胶质细胞酸性蛋白（GFAP）作为增殖、迁移和分化的标志物。溴脱氧尿苷标记的细胞增加约7倍，PSA-NCAM阳性细胞增加约3倍，在颗粒下区（SGZ），缺血后10天达到高峰。缺血后20 d，SGZ和颗粒细胞层（GCL）中首次检测到表达PSA-NCAM的溴脱氧尿苷标记细胞，此后逐渐减少，而表达NeuN的BrdU标记细胞在GCL中逐渐增加，直至缺血后60 d。缺血后DG内有少量BrdU标记的GFAP阳性细胞，未见PSA-NCAM阳性细胞表达GFAP，但胶质细胞放射状突起部分与PSA-NCAM阳性胞体和树突接触。这些结果表明，神经干细胞增殖开始于SGZ，然后细胞迁移到GCL并主要分化为神经元细胞。这三个步骤大多数在短暂性全脑缺血后2个月内完成。
The stage of neurogenesis can be divided into three steps: proliferation, migration, and differentiation. To elucidate detailed relations between these three steps after ischemia, the authors evaluated the three steps in the adult gerbil dentate gyrus (DG) after 5 minutes of transient global ischemia using bromodeoxyuridine (BrdU), highly polysialylated neural cell adhesion molecule (PSA-NCAM), and neuronal nuclear antigen (NeuN) and glial fibrillary acidic protein (GFAP) as markers for proliferation, migration, and differentiation, respectively. Bromodeoxyuridine-labeled cells increased approximately sevenfold, and PSA-NCAM-positive cells increased approximately threefold in the subgranular zone (SGZ) with a peak 10 days after ischemia. Bromodeoxyuridine-labeled cells with PSA-NCAM expression were first detected both in the SGZ and the granule cell layer (GCL) 20 days after ischemia and gradually decreased after that, whereas BrdU-labeled cells with NeuN gradually increased in the GCL until 60 days after ischemia. A few BrdU-Iabeled cells with GFAP expression were detected in DG after ischemia; no PSA-NCAM-positive cells with GFAP expression were detected, but the radial processes of glial cells were partly in contact with PSA-NCAM-positive cell bodies and dendrites. These results suggest that neural stem cell proliferation begins at the SGZ, and that the cells then migrate into the GCL and differentiate mainly into neuronal cells. The majority of these three steps finished in 2 months after transient global ischemia.