POLYMERIC IMMUNOGLOBULIN RECEPTOR EXPRESSED IN MDCK CELLS TRANSCYTOSES IGA
POLYMERIC IMMUNOGLOBULIN RECEPTOR EXPRESSED IN MDCK CELLS TRANSCYTOSES IGA
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DOI：
10.1016/0092-8674(86)90887-1
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发表时间：
1986-08-15
期刊：
影响因子：
64.5
通讯作者：
DEITCHER, DL
中科院分区：
文献类型：
--
作者：
MOSTOV, KE;DEITCHER, DL
我们在极化的Madin-Darby犬肾上皮细胞中表达了兔多聚免疫球蛋白受体的cDNA，而正常情况下Madin-Darby犬肾上皮细胞不产生这种受体。受体的功能似乎与体内一样；二聚体IgA从基底外侧运输到根尖表面，并与受体的裂解片段一起释放到根尖介质中，称为分泌成分。这一系统首次使我们能够在体外定量研究免疫球蛋白A的跨细胞作用，从而进行以下观察。首先，新制造的受体中90%最终被切割成分泌成分并释放到根尖介质中，首先进入基底外侧表面。其次，受体的转运不依赖于配体结合。第三，结合配体的跨细胞反应的半衰期为30分钟。
We expressed cDNA for the rabbit polymeric immunoglobulin receptor in polarized Madin-Darby Canine Kidney epithelial cells, which normally do not produce this receptor. The receptor appeared to function as in vivo; dimeric IgA was transported from the basolateral to the apical surface and released into the apical medium, together with the cleaved fragment of the receptor, known as secretory component. This system enabled us, for the first time, to study quantitatively IgA transcytosis in vitro and thus make the following observations. First, > 90% of the newly made receptor that is ultimately cleaved to secretory component and released into the apical medium goes first to the basolateral surface. Second, transport of the receptor does not depend on ligand binding. Third, transcytosis of bound ligand has a t1/2 of 30 min.