Hydrogen production characteristics of photoheterotrophic Rubrivivax gelatinosus L31
Hydrogen production characteristics of photoheterotrophic Rubrivivax gelatinosus L31
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DOI：
10.1016/j.ijhydene.2007.12.001
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发表时间：
2008-02
影响因子：
7.2
通讯作者：
Ru Ying Li;H. H. Fang-H.
中科院分区：
文献类型：
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作者：
Ru Ying Li;H. H. Fang-H.
从某水库底泥中分离到一株胶状红病毒（Rubrivivax gelatinosus），命名为L31。对10种有机底物进行测试，该菌株可以从碳水化合物（包括葡萄糖、蔗糖和淀粉）以及乳酸盐和苹果酸盐中产生氢气。虽然它可以利用乙酸、丙酸、丁酸、琥珀酸和谷氨酸作为底物，但不能从它们产生氢。根据实验数据确定的动力学参数，乳酸产氢量最高（225.4ml），氢转化率为50.5%，而淀粉在870 h的长滞后期后表现出最高的产氢速率（829 ml/g/h）。固氮酶活性在9.0 ~ 36.3μ l-C_2 H_4/h/mg-VSS范围内增加，通常导致更高的底物降解和氢转化效率。虽然氢和细胞内聚-β-羟基丁酸酯的形成都消耗电子，但它们之间没有明显的定量相关性。
A new strain of Rubrivivax gelatinosus (designated as L31) was isolated from the sediment of a local reservoir. Testing against 10 organic substrates, this strain could produce hydrogen from carbohydrates, including glucose, sucrose and starch, as well as from lactate and malate. Even though it could use acetate, propionate, butyrate, succinate and glutamate as substrate, it could not produce hydrogen from them. Based on the determined kinetic parameters derived from experimental data, lactate produced the highest amount (225.4ml) of hydrogen with a hydrogen conversion efficiency of 50.5%, whereas starch exhibited the highest production rate of 829ml/g/h after an extensive lag phase of 870h. The increase of nitrogenase activity, which ranged from 9.0 to 36.3μl-C2H4/h/mg-VSS, generally resulted in higher substrate degradation and hydrogen conversion efficiency. Although both formations of hydrogen and intracellular poly-β-hydroxybutyrate consumed electrons, there was no noticeable quantitative correlation between them.