Different Metabolic Responses of Human Brown Adipose Tissue to Activation by Cold and Insulin
Different Metabolic Responses of Human Brown Adipose Tissue to Activation by Cold and Insulin
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DOI：
10.1016/j.cmet.2011.06.012
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发表时间：
2011-08-03
期刊：
影响因子：
29
通讯作者：
Virtanen, Kirsi A.
中科院分区：
文献类型：
--
作者：
Orava, Janne;Nuutila, Pirjo;Virtanen, Kirsi A.
我们研究了人类棕色脂肪组织（BAT）在健康受试者的代谢，通过确定其冷诱导和胰岛素刺激的葡萄糖摄取和血流量（灌注），使用正电子发射断层扫描（PET）结合计算机断层扫描（CT）。其次，我们评估了人类BAT和白色脂肪组织（WAT）中的基因表达。葡萄糖摄取诱导12倍BAT冷，伴随着加倍的灌注。我们发现全身能量消耗和BAT灌注之间存在正相关。胰岛素增强葡萄糖摄取5倍BAT独立于其灌注，而对WAT的影响较弱。胰岛素敏感性葡萄糖转运蛋白GLUT 4的基因表达水平在BAT中也高于WAT。总之，BAT似乎被胰岛素和冷不同地激活;响应于胰岛素，BAT显示高葡萄糖摄取而不增加灌注，但是当被冷激活时，它以灌注依赖性方式耗散能量。
We investigated the metabolism of human brown adipose tissue (BAT) in healthy subjects by determining its cold-induced and insulin-stimulated glucose uptake and blood flow (perfusion) using positron emission tomography (PET) combined with computed tomography (CT). Second, we assessed gene expression in human BAT and white adipose tissue (WAT). Glucose uptake was induced 12-fold in BAT by cold, accompanied by doubling of perfusion. We found a positive association between whole-body energy expenditure and BAT perfusion. Insulin enhanced glucose uptake 5-fold in BAT independently of its perfusion, while the effect on WAT was weaker. The gene expression level of insulin-sensitive glucose transporter GLUT4 was also higher in BAT as compared to WAT. In conclusion, BAT appears to be differently activated by insulin and cold; in response to insulin, BAT displays high glucose uptake without increased perfusion, but when activated by cold, it dissipates energy in a perfusion-dependent manner.