Avian influenza virus (H5N1); effects of physico-chemical factors on its survival.
Avian influenza virus (H5N1); effects of physico-chemical factors on its survival.
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DOI：
10.1186/1743-422x-6-38
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发表时间：
2009-03-28
期刊：
影响因子：
4.8
通讯作者：
Hassan S
中科院分区：
文献类型：
--
作者：
Shahid MA;Abubakar M;Hameed S;Hassan S
本研究旨在确定物理和化学制剂对高致病性禽流感（HPAI） H5N1（当地毒株）病毒感染潜力的影响，该病毒是2006年在巴基斯坦暴发期间最近分离出来的。将滴度为108.3 ELD50/ml的H5N1病毒与无菌的尿水混合，得到4HA单位的最终稀释，然后暴露于物理（温度、pH和紫外光）和化学（福尔马林、苯酚晶体、碘晶体、CID 20、virkon®-S、zeptin 10%、KEPCIDE 300、KEPCIDE 400、lifebuoy、surf excel和碱）剂中。从接种H5N1处理过的病毒（0.2 ml/蛋）的胚鸡蛋中收集羊水-尿囊液（AAF），进行血凝（HA）和血凝抑制（HI）试验。H5N1病毒在56°C条件下30分钟后失去传染性，在28°C条件下1天后失去传染性，但在4°C条件下仍可存活100多天。接触6 h后，酸性pH（1、3）和碱性pH（11、13）对病毒有杀菌作用；但在pH值为5 （18 h）、7和9 （24 h以上）时，病毒仍具有传染性。紫外线照射60分钟后也不能完全灭活病毒。肥皂（lifebuoy®）、洗涤剂（surf excel®）和碱（烧碱）在0.1、0.2和0.3%的稀释下，在5分钟后破坏传染性。所有市售消毒剂均按推荐浓度灭活病毒。本研究结果将有助于在禽流感爆发后在养殖场/孵化场层面实施生物安全措施。
Present study was performed to determine the effects of physical and chemical agents on infective potential of highly pathogenic avian influenza (HPAI) H5N1 (local strain) virus recently isolated in Pakistan during 2006 outbreak. H5N1 virus having titer 108.3 ELD50/ml was mixed with sterilized peptone water to get final dilution of 4HA units and then exposed to physical (temperature, pH and ultraviolet light) and chemical (formalin, phenol crystals, iodine crystals, CID 20, virkon®-S, zeptin 10%, KEPCIDE 300, KEPCIDE 400, lifebuoy, surf excel and caustic soda) agents. Harvested amnio-allantoic fluid (AAF) from embryonated chicken eggs inoculated with H5N1 treated virus (0.2 ml/egg) was subjected to haemagglutination (HA) and haemagglutination inhibition (HI) tests. H5N1 virus lost infectivity after 30 min at 56°C, after 1 day at 28°C but remained viable for more than 100 days at 4°C. Acidic pH (1, 3) and basic pH (11, 13) were virucidal after 6 h contact time; however virus retained infectivity at pH 5 (18 h), 7 and 9 (more than 24 h). UV light was proved ineffectual in inactivating virus completely even after 60 min. Soap (lifebuoy®), detergent (surf excel®) and alkali (caustic soda) destroyed infectivity after 5 min at 0.1, 0.2 and 0.3% dilution. All commercially available disinfectants inactivated virus at recommended concentrations. Results of present study would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake of avian influenza virus (AIV) outbreak.