Virazole prevents production of Sindbis virus and virus-induced cytopathic effect in Aedes albopictus cells.
Virazole prevents production of Sindbis virus and virus-induced cytopathic effect in Aedes albopictus cells.
复制标题
病毒唑可防止白纹伊蚊细胞中辛德比斯病毒的产生和病毒诱导的细胞病变效应。
DOI：
10.1016/0042-6822(78)90375-6
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发表时间：
1978
期刊：
影响因子：
3.7
通讯作者：
V. Stollar
中科院分区：
文献类型：
--
作者：
N. Sarver;V. Stollar
The nucleoside analogue virazole (1-β-d-ribofuranosyl 1,2,4-triazole-3-carboxamide) at a concentration of 150 μg/ml inhibited the production of Sindbis virus (SV) and prevented virus-induced cytopathic effect (CPE) inAedes albopictusLT C-7 cells. The drug had no effect on net viral RNA synthesis, the production of infectious RNA, or on nucleocapsid assembly. All viral structural proteins (E1, E2, and C) were synthesized and glycosylated in apparently normal amounts in the presence of virazole (Vz). However, most cells contained little or no reactive viral antigens as measured by an indirect immunofluorescent test. In contrast to the minimal effect on the synthesis of viral RNA and proteins, Vz markedly inhibited host macromolecular synthesis, as well as the growth of mosquito cells. In BHK21 cells the situation was different. As in C-7 cells, the drug caused a marked reduction in host macromolecular synthesis. However, CPE was not prevented and virus production was not inhibited. It is suggested that certain host functions may be required in the late stages of viral maturation and for expression of CPE and that these host functions are inhibited by Vz.
