The effect of local passive heating on skeletal muscle histamine concentration: implications for exercise-induced histamine release.
The effect of local passive heating on skeletal muscle histamine concentration: implications for exercise-induced histamine release.
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局部被动加热对骨骼肌组胺浓度的影响：对运动引起的组胺释放的影响。
DOI：
10.1152/japplphysiol.00740.2021
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Halliwill,JohnR
中科院分区：
文献类型：
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作者：
Mangum,JoshuaE;Needham,KarenWiedenfeld;Sieck,DylanC;Ely,MatthewR;Larson,EmilyA;Peck,MairinC;Minson,ChristopherT;Halliwill,JohnR
有氧运动诱导肥大细胞脱颗粒，并通过组氨酸脱羧酶增加组胺的形成，导致肌内组胺增加~ 150%。这项研究的目的是确定与运动相关的骨骼肌温度的升高是否足以解释这种组胺反应。具体来说，我们假设局部被动加热模仿运动中观察到的骨骼肌温度变化的幅度和时间过程，会导致肌内组胺浓度增加，与运动值相当。主要研究采用脉冲短波热疗法被动升高股外侧肌温度60 min。加热使肌内温度从32.6°C[95%可信区间（CI） 32.0°C ~ 33.2°C]升高至38.9°C(38.7°C ~ 39.2°C) (P< 0.05)，使肌内组胺浓度从2.14 ng/mL （1.92 ~ 2.36 ng/mL）升高至2.97 ng/mL (2.57 ~ 3.36 ng/mL) (P< 0.05)，升高41%。在使用人源性培养肥大细胞的后续体外实验中，加热到相当的温度并没有激活肥大细胞脱颗粒。因此，运动相关的骨骼肌温度变化足以引起肌内组胺浓度升高。然而，这种热效应最有可能是由于组氨酸脱羧酶引起的新生组胺形成的变化，而不是肥大细胞的脱颗粒。总之，骨骼肌温度的生理相关升高部分解释了组胺对有氧运动的反应，但不是全部。这种热效应对于产生对运动训练的积极适应可能很重要。在有氧运动中，激活骨骼肌释放组胺的“运动信号”尚不清楚。通过模拟在有氧运动中观察到的骨骼肌温度升高的幅度和时间过程，我们证明了运动引起的组胺升高的部分原因是热效应，体外实验表明这很可能是通过重新形成组胺。这种热效应对于产生对运动训练的积极适应可能很重要。
Aerobic exercise induces mast cell degranulation and increases histamine formation by histidine decarboxylase, resulting in an ∼150% increase in intramuscular histamine. The purpose of this study was to determine if the increase in skeletal muscle temperature associated with exercise is sufficient to explain this histamine response. Specifically, we hypothesized that local passive heating that mimics the magnitude and time course of changes in skeletal muscle temperature observed during exercise would result in increased intramuscular histamine concentrations comparable to exercising values. Seven subjects participated in the main study in which pulsed short-wave diathermy was used to passively raise the temperature of the vastus lateralis over 60 min. Heating increased intramuscular temperature from 32.6°C [95% confidence interval (CI) 32.0°C to 33.2°C] to 38.9°C (38.7°C to 39.2°C) (P< 0.05) and increased intramuscular histamine concentration from 2.14 ng/mL (1.92 to 2.36 ng/mL) to 2.97 ng/mL (2.57 to 3.36 ng/mL) (P< 0.05), an increase of 41%. In a follow-up in vitro experiment using human-derived cultured mast cells, heating to comparable temperatures did not activate mast cell degranulation. Therefore, it appears that exercise-associated changes in skeletal muscle temperature are sufficient to generate elevations in intramuscular histamine concentration. However, this thermal effect is most likely due to changes in de novo histamine formation via histidine decarboxylase and not due to degranulation of mast cells. In conclusion, physiologically relevant increases in skeletal muscle temperature explain part, but not all, of the histamine response to aerobic exercise. This thermal effect may be important in generating positive adaptations to exercise training.NEW & NOTEWORTHYThe “exercise signal” that triggers histamine release within active skeletal muscle during aerobic exercise is unknown. By mimicking the magnitude and time course of increasing skeletal muscle temperature observed during aerobic exercise, we demonstrate that part of the exercise-induced rise in histamine is explained by a thermal effect, with in vitro experiments suggesting this is most likely via de novo histamine formation. This thermal effect may be important in generating positive adaptations to exercise training.