Resident-intruder trait aggression is associated with differences in lysine vasopressin and serotonin receptor 1A (5-HT1A) mRNA expression in the brain of pre-pubertal female domestic pigs (Sus scrofa)
Resident-intruder trait aggression is associated with differences in lysine vasopressin and serotonin receptor 1A (5-HT1A) mRNA expression in the brain of pre-pubertal female domestic pigs (Sus scrofa)
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DOI：
10.1111/j.1365-2826.2005.01359.x
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发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Meddle, SL
中科院分区：
文献类型：
--
作者：
D'Eath, RB;Ormandy, E;Meddle, SL
家猪在遇到陌生个体后表现出的攻击性行为是一个严重的福利和经济问题。导致皮肤损伤的攻击行为在青春期前的猪中同样普遍，无论性别如何。人们对猪控制攻击性的神经回路和神经肽知之甚少。由于有证据表明加压素和血清素能系统参与雄性哺乳动物攻击行为的调节，因此我们利用定量原位杂交技术，对攻击性和非攻击性青春期前雌性猪的特定脑区域内加压素和血清素 1A 受体 (5-HT1A) mRNA 表达进行定量原位杂交，以寻找差异。攻击性猪的内侧杏仁核、外侧隔膜 (LS) 中表达加压素 mRNA 的细胞数量显着较高，并且在终纹床核 (BnST) 中表现出类似的趋势，但在室旁核 (PVN) 或视上核中则没有。 5-HT1A 受体在猪脑中广泛表达，并且在 BnST 中观察到 5-HT1A mRNA 表达强度（银粒密度）显着降低。在攻击性猪的内侧杏仁核和 LS 中，表达 5-HT1A mRNA 的细胞较少，但在 PVN 中没有发现差异。在没有近交系或选择系的情况下，这些发现表明，在神经研究之前预先识别群体中的表型行为极端是一种有用的技术。此外，这些发现支持加压素和血清素在青春期前雌性猪的高性状攻击性调节中的核心作用。
Aggressive behaviour exhibited by domestic pigs following encounters with unfamiliar individuals is a serious welfare and economical problem. Aggression resulting in skin lesions is similarly prevalent in prepubertal pigs of either sex. Little is known about the neural circuits and neuropeptides that control aggression in the pig. Because there is evidence for the involvement of the vasopressin and serotonergic systems in the regulation of aggressive behaviour in male mammals, we sought differences using quantitative in situ hybridisation of vasopressin and serotonin 1A receptor (5-HT1A) mRNA expression within specific brain regions of aggressive and nonaggressive prepubertal female pigs. The number of cells expressing vasopressin mRNA was significantly higher in aggressive pigs in the medial amygdala, lateral septum (LS) and showed a similar trend in the bed nucleus of the stria terminalis (BnST) but not the paraventricular nucleus (PVN) or supraoptic nucleus. The 5-HT1A receptor was widely expressed through the porcine brain and a significantly lower intensity (silver grain density) of 5-HT1A mRNA expression was observed in the BnST. In the medial amygdala and LS fewer cells expressed 5-HT1A mRNA in aggressive pigs but no differences were found in the PVN. In the absence of inbred strains or selection lines, these findings have shown that prior identification of phenotypic behavioural extremes in a population in advance of neural studies is a useful technique. Moreover, these findings support a central role for vasopressin and serotonin in the mediation of high trait aggression in prepubertal female pigs.