Micropropagation of Chinquapin (Castanea henryi) Using Axillary Shoots and Cotyledonary Nodes
Micropropagation of Chinquapin (Castanea henryi) Using Axillary Shoots and Cotyledonary Nodes
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DOI：
10.21273/hortsci13292-18
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发表时间：
2018-10
期刊：
影响因子：
1.9
通讯作者：
H. Xiong;He Sun;F. Zou;Xiao-Ming Fan;G. Niu;D. Yuan
中科院分区：
文献类型：
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作者：
H. Xiong;He Sun;F. Zou;Xiao-Ming Fan;G. Niu;D. Yuan
锥栗是我国重要的木本粮食树种。本研究的目的是寻找适宜的植物生长调节剂（PGRs）和最佳浓度，直接器官发生腋芽和子叶节。从田间收集种子，灭菌，并在体外发芽。以3周龄幼苗的腋芽和子叶节为外植体。在MS +0.65%琼脂+3%蔗糖的培养基中添加0.5mg·L-1 6-苄基氨基嘌呤（6-BA）、0.1mg·L-1吲哚乙酸（IAA）、0.1mg·L-1 2，4-二氯苯氧乙酸（2，4-D）或0.1mg·L-1萘乙酸（NAA），可获得诱导不定芽的最佳培养基。在子叶节中添加0.1mg·L-12，4-D可获得较高的不定芽诱导率（85.67%）。外植体类型影响不定芽增殖率和质量。顶端外植体比节段产生更多和更长的芽。L-1 6-BA + 0.05 mg·L-1吲哚-3-丁酸（IBA）+MS培养基上，茎尖和节段分别产生12.33和6.25个不定芽。L-16-BA +0.05mg·L-1 IBA + MS培养基对不定芽的伸长和壮苗效果最好，不定芽平均长3.50cm，粗0.46cm，叶片数8片左右。在MS +3.5%NAA +1.5mg·L-1 IBA的培养基上，生根率最高，分别为76.70%和11.33条/枝。生根苗在温室中驯化，成活率达80%。从扩繁到驯化，有助于实现锥栗优质树的大量繁殖，提高产量和坚果品质。
Castanea henryi is an important woody grain tree species native to China. The objective of the current study was to find the suitable plant growth regulators (PGRs) and the optimal concentrations for direct organogenesis by using axillary shoots and cotyledonary nodes. Seeds were collected from the field, sterilized, and germinated in vitro. Axillary shoots and cotyledonary nodes of 3-week-old seedlings were used as explants. To find the suitable PGR for adventitious shoot induction, 0.5 mg·L–1 6-benzylaminopurine (6-BA), 0.1 mg·L–1 indole-3-acetic acid (IAA), 0.1 mg·L–1 2,4-dichlorophenoxyacetic acid (2,4-D), or 0.1 mg·L–1 1-naphthaleneacetic acid (NAA) was supplemented to Murashige and Skoog (MS) medium containing 0.65% agar and 3% sucrose. A high induction percentage of adventitious shoots (85.67%) was obtained from cotyledonary nodes supplemented with 0.1 mg·L–1 2,4-D. The type of explant influenced shoot proliferation rates and quality. Apical explants produced more and longer shoots than nodal segments. For shoot multiplication, 1 mg·L–1 6-BA + 0.05 mg·L–1 indole-3-butyric acid (IBA) supplemented with MS medium produced 12.33 and 6.25 shoots per explant, respectively, from apical and nodal explants. For shoot elongation and strengthening, 2 mg·L–1 6-BA + 0.05 mg·L–1 IBA supplemented with MS medium was the best combination, producing shoots with a mean length of 3.50 cm, a diameter of 0.46 cm, and about eight leaves per shoot. The greatest rooting of 76.70% and 11.33 roots per shoot was achieved when cultured in MS medium supplemented with 3.5% perlite + 1.5 mg·L–1 IBA. For acclimatization of the rooted plantlets in the greenhouse, a survival rate of 80% was achieved. This protocol—from multiplication to acclimation—is helpful to realize mass propagation of high-quality trees of chinquapin for increasing production and nut quality.