THE V(D)J RECOMBINATION ACTIVATING GENE, RAG-1
THE V(D)J RECOMBINATION ACTIVATING GENE, RAG-1
复制标题
DOI：
10.1016/0092-8674(89)90760-5
复制
发表时间：
1989-12-22
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
SCHATZ, DG;OETTINGER, MA;BALTIMORE, D
RAG-1（重组激活基因-1）基因组位点，其在引入NIH 3 T3成纤维细胞时激活V（D）J重组，通过连续基因组转染阿托伐他汀标记的DNA来分离。跨越55 kb的基因组步移产生RAG-1基因组探针，其检测转染子和前B细胞和前T细胞中的单个6.6-7.0 kb mRNA种类。RAG-1基因组和cDNA克隆在NIH 3 T3细胞中具有生物活性。人和小鼠RAG-1 cDNA克隆的核苷酸测序分别预测119 kd的1043和1040个氨基酸的蛋白质，具有90%的序列同一性。RAG-1在进行V（D）J重组的物种之间是保守的，并且其表达模式与V（D）J重组酶活性的表达模式完全相关。RAG-1可以间接激活V（D）J重组，或者它可以编码V（D）J重组酶本身。
The RAG-1 (recombination activating gene-1) genomic locus, which activates V(D)J recombination when introduced into NIH 3T3 fibroblasts, was isolated by serial genomic transfections of oligonucleotide-tagged DNA. A genomic walk spanning 55 kb yielded a RAG-1 genomic probe that detects a single 6.6-7.0 kb mRNA species in transfectants and pre-B and pre-T cells. RAG-1 genomic and cDNA clones were biologically active when introduced into NIH 3T3 cells. Nucleotide sequencing of human and mouse RAG-1 cDNA clones predicts 119 kd proteins of 1043 and 1040 amino acids, respectively, with 90% sequence identity. RAG-1 has been conserved between species that carry out V(D)J recombination, and its pattern of expression correlates exactly with the pattern of expression of V(D)J recombinase activity. RAG-1 may activate V(D)J recombination indirectly, or it may encode the V(D)J recombinase itself.