Fabrication and characterization of electrospun osteon mimicking scaffolds for bone tissue engineering
Fabrication and characterization of electrospun osteon mimicking scaffolds for bone tissue engineering
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DOI：
10.1016/j.msec.2010.10.001
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发表时间：
2011-01-01
影响因子：
7.9
通讯作者：
Freeman, J. W.
中科院分区：
文献类型：
--
作者：
Andric, T.;Sampson, A. C.;Freeman, J. W.
骨骼丢失和骨质缺乏是一个全球性的重大问题，仅在美国每年就进行超过 600,000 例手术，使骨骼成为移植最多的组织之一，仅次于血液。骨是由有机基质、无机骨矿物质和水组成的复合组织。从结构上讲，骨分为两种不同的类型：小梁骨（或松质骨）和皮质骨（或致密骨）。骨小梁的特征是广泛互连的孔隙网络。皮质骨由紧密排列的单位（称为骨单位）组成，其方向平行于骨轴。虽然大多数支架试图复制小梁骨的结构，但很少有人尝试创建模拟皮质骨结构的支架。这项研究的目的是开发一种技术来制造模仿骨组织（皮质骨的结构单元）的支架。我们成功地构建了 PGA 纤维的旋转台，并利用它来收集静电纺丝纳米纤维并创建支架。所得支架由电纺 PLLA 或明胶/PLLA 纳米纤维的同心层组成，这些纤维包裹在 PGA 微纤维核心上，直径范围为 200 至 600 μm。支架通过在 10 倍模拟体液中孵育而矿化，由 10% 明胶/PLLA 组成的支架具有明显更高含量的磷酸钙。电纺支架还支持 MC3T3 细胞在 28 天的时间内附着和增殖。由 Elsevier B.V. 出版
Skeletal loss and bone deficiencies are a major worldwide problem with over 600,000 procedures performed in the US alone annually, making bone one of the most transplanted tissues, second to blood only. Bone is a composite tissue composed of organic matrix, inorganic bone mineral, and water. Structurally bone is organized into two distinct types: trabecular (or cancellous) and cortical (or compact) bones. Trabecular bone is characterized by an extensive interconnected network of pores. Cortical bone is composed of tightly packed units, called osteons, oriented parallel along to the axis of the bone. While the majority of scaffolds attempt to replicate the structure of the trabecular bone, fewer attempts have been made to create scaffolds to mimic the structure of cortical bone. The aim of this study was to develop a technique to fabricate scaffolds that mimic the organization of an osteon, the structural unit of cortical bone. We successfully built a rotating stage for PGA fibers and utilized it for collecting electrospun nanofibers and creating scaffolds. Resulting scaffolds consisted of concentric layers of electrospun PLLA or gelatin/PLLA nanofibers wrapped around PGA microfiber core with diameters that ranged from 200 to 600 mu m. Scaffolds were mineralized by incubation in 10x simulated body fluid, and scaffolds composed of 10%gelatin/PLLA had significantly higher amounts of calcium phosphate. The electrospun scaffolds also supported cellular attachment and proliferation of MC3T3 cells over the period of 28 days. Published by Elsevier B.V.