On the mechanism of malonyl-CoA-independent fatty-acid synthesis. Characterization of the mitochondrial chain-elongating system of rat liver and pig-kidney cortex.
On the mechanism of malonyl-CoA-independent fatty-acid synthesis. Characterization of the mitochondrial chain-elongating system of rat liver and pig-kidney cortex.
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关于不依赖丙二酰辅酶A的脂肪酸合成机制。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
W. Seubert
中科院分区：
文献类型：
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作者：
W. Hinsch;W. Seubert
1.大鼠肝和猪肾皮质线粒体丙酮粉提取物对脂肪酸链的延长作用具有相似的性质。与肝脏系统2相比，肾脏系统的比活性约为30%。文献中报道的在NADH作为唯一氢供体的情况下，[1-14-C]醋酸盐向脂肪酸中的不同掺入率可以通过不同的提取方法来解释。3.在肝脏中，掺入饱和脂肪酸，延长一个C-2单位，与两种核苷酸存在下的掺入相比，NADH和NADPH的掺入量仅为19%和60%。4.链延长过程的动力学有利于烯酰辅酶A还原酶成为限速步骤。5.长链饱和和不饱和脂肪酰辅酶A衍生物是链延长的非常差的引物。6. Possion和烯酰辅酶A还原酶可能是将氢从NADPH转移到呼吸链，以及在细胞缺氧期间还原当量（NADH和NADPH）或乙酸单位的保存。
1. Chain elongation of fatty acids by extracts of mitochondrial acetone powders from rat liver and pig kidney cortex are similar in their properties. The specific activity of the kidney system is about 30% as compared to the liver system 2. Different incorporation rates [1-14-C] acetate into fatty acids in the presence of NADH as the sole hydrogen donor that were reported in literature can be explained by different extraction methods. 3. In liver the incorporation into the saturated fatty acid, elongated by one C-2 unit, amounts to only 19% with NADH and 60% with NADPH in comparison with the incorporation in presence of both nucleotides. 4. Kinetics of the chain-elongating process favour the enoyl-CoA reductase to be the rate limiting step. 5. Long-chain saturated and unsaturated fatty acyl-CoA derivatives are very poor primers of the chain elongation. 6. Possion and the enoyl-CoA reductase may be the transfer of hydrogen from NADPH to the respiratory chain, and the conservation of reducing equivalents (NADH and NADPH) or acetate units during cellular anoxia.