Histological and transcriptome analyses of testes from Duroc and Meishan boars.
Histological and transcriptome analyses of testes from Duroc and Meishan boars.
复制标题
杜洛克和梅山公猪睾丸的组织学和转录组分析
DOI：
10.1038/srep20758
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发表时间：
2016-02-11
影响因子：
4.6
通讯作者：
Xu D
中科院分区：
文献类型：
--
作者：
Ding H;Luo Y;Liu M;Huang J;Xu D
梅山野猪以性成熟早而闻名。然而，他们表现出一个显着较小的睾丸大小和支持细胞的比例减少，每天精子产量与杜洛克公猪相比。对20、75和270日龄杜洛克公猪和梅山公猪的睾丸进行组织学和转录组学分析。采用苏木精-伊红染色法对不同日龄杜洛克猪和梅山猪睾丸的组织结构进行了观察。梅山猪的精子发生发生在75日龄以前，但成年期的精原细胞和支持细胞数量明显少于杜洛克猪。两个品种睾丸生精小管的直径在生精小管发育的起始阶段存在显著差异。我们获得了差异表达的功能基因，并使用RNA-seq分析了参与男性性成熟和精子发生的七条途径。我们还发现了四个主要的选择性剪接事件和许多单核苷酸多态性从睾丸。通过qPCR验证八个功能重要的基因，并对神经营养因子3进行定量和细胞定位分析。本研究为梅山猪和杜洛克猪性功能发育差异提供了第一个转录组证据。
Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars.