N‐methyl‐D‐aspartate receptor blockade inhibits estrogenic support of dendritic growth in a sexually dimorphic rat spinal nucleus
N‐methyl‐D‐aspartate receptor blockade inhibits estrogenic support of dendritic growth in a sexually dimorphic rat spinal nucleus
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N-甲基-D-天冬氨酸受体阻断抑制性别二态性大鼠脊髓核中树突生长的雌激素支持
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Sengelaub
中科院分区：
文献类型：
--
作者：
S. L. Hebbeler;T. Verhovshek;D. Sengelaub
大鼠腰髓内含性二态、类固醇敏感的球海绵体脊髓核(SNB)。SNB运动神经元的树突发育需要雄激素和雌激素的共同作用。雌激素效应仅限于SNB树突在4周龄时的初始生长。在出生后，其他脊髓运动神经元中的树突生长受N-甲基-D-天冬氨酸(NMDA)受体激活的调节。在这项研究中，我们测试了NMDA受体激活是否参与SNB树突状细胞的生长，以及SNB树突状细胞生长的雌激素支持是否依赖于NMDA受体的激活。对正常男性、每天接受NMDA受体拮抗剂MK-801治疗的完整男性、接受苯甲酸雌二醇(EB)治疗的去势男性以及同时接受EB和MK-801治疗的去势男性的运动神经元形态进行了评估。用霍乱毒素-辣根过氧化物酶逆行标记4周龄SNB运动神经元(树突长度通常最大)，并进行三维重建。经MK-801处理的正常雄性SNB运动神经元的胞体面积和树突长度均低于正常雄性。EB处理组大鼠的树突状生长得到部分支持，但MK-801处理组的树突状细胞生长被阻断。这些结果表明，与其他运动神经元一样，SNB的树突发育涉及NMDA受体，此外，SNB树突发育中对雌激素敏感的成分需要它们的激活。J.Comp.神经。451：142-152,2002。©2002 Wiley-Liss公司
The lumbar spinal cord of rats contains the sexually dimorphic, steroid‐sensitive spinal nucleus of the bulbocavernosus (SNB). Dendritic development of SNB motoneurons requires the action of both androgens and estrogens. Estrogenic effects are limited to the initial growth of SNB dendrites through 4 weeks of age. During this postnatal period, dendritic growth in other spinal motoneurons is regulated by N‐methyl‐D‐aspartate (NMDA) receptor activation. In this study, we tested whether NMDA receptor activation was involved in SNB dendritic growth and whether the estrogenic support of SNB dendritic growth was dependent on the activation of NMDA receptors. Motoneuron morphology was assessed in normal males, intact males treated daily with the NMDA receptor antagonist MK‐801, castrated males treated with estradiol benzoate (EB), and castrated males treated with both EB and MK‐801. SNB motoneurons were retrogradely labeled with cholera toxin‐horseradish peroxidase at 4 weeks of age (when dendritic length is normally maximal) and reconstructed in three dimensions. Somal area and dendritic length of SNB motoneurons in MK‐801‐treated, intact males were below those of normal males. Dendritic growth was partially supported in EB‐treated castrates, but this growth was blocked by MK‐801 treatment. These results suggest that, as in other motoneurons, dendritic development in the SNB involves NMDA receptors and, furthermore, that the estrogen‐sensitive component of SNB dendritic development requires their activation. J. Comp. Neurol. 451:142–152, 2002. © 2002 Wiley‐Liss, Inc.
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卵巢切除术减弱了激素敏感的脊髓运动神经元的树突生长。
DOI：
10.1002/neu.1059
发表时间：
2001
期刊：
Journal of neurobiology.
影响因子：
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作者：
Hebbeler,SL;Verhovshek,T;Sengelaub,DR
通讯作者：
Sengelaub,DR
星形胶质细胞诱导培养的交感神经元的树突发育。
DOI：
10.1016/0165-3806(89)90184-3
发表时间：
1989
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Johnson,MI;Higgins,D;Ard,MD
通讯作者：
Ard,MD
影响因子：
4.6
作者：
R. Kalb
通讯作者：
R. Kalb
DOI：
10.1016/0165-3806(93)90133-u
发表时间：
1993-06-08
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
GOLDSTEIN, LA;KURZ, EM;SENGELAUB, DR
通讯作者：
SENGELAUB, DR