Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications
Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications
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DOI：
10.1021/acsami.9b00666
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发表时间：
2019-05-22
影响因子：
9.5
通讯作者：
Mestroni, Luisa
中科院分区：
文献类型：
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作者：
Pena, Brisa;Maldonado, Marcos;Mestroni, Luisa
心肌梗死后心脏功能的恢复与严重的发病率和死亡率相关，利用心脏组织工程中的聚合物有望。为了正确模拟天然心脏组织，材料不仅必须支持心脏细胞生长，而且还必须具有固有的导电性能。在这里，我们提出了一种可注射的反向热凝胶（RTG）为基础的心脏细胞支架系统，既生物相容性和导电性。在合成高度官能化的仿生RTG骨架之后，将金纳米颗粒（AuNPs）化学缀合到骨架以增强系统的导电性。当与心脏成纤维细胞共培养时，所得的RTG-AuNP水凝胶支持新生大鼠心室肌细胞（NRVM）的靶向存活长达21天，导致连接蛋白43（Cx43）相对于对照培养物（在传统明胶涂覆的培养皿和不含AuNP的RTG水凝胶上培养的NRVM）增加。这种仿生和导电RTG-AuNP水凝胶为未来的心脏组织工程应用提供了希望。
Utilizing polymers in cardiac tissue engineering holds promise for restoring function to the heart following myocardial infarction, which is associated with grave morbidity and mortality. To properly mimic native cardiac tissue, materials must not only support cardiac cell growth but also have inherent conductive properties. Here, we present an injectable reverse thermal gel (RTG)-based cardiac cell scaffold system that is both biocompatible and conductive. Following the synthesis of a highly functionalizable, biomimetic RTG backbone, gold nanoparticles (AuNPs) were chemically conjugated to the backbone to enhance the system's conductivity. The resulting RTG-AuNP hydrogel supported targeted survival of neonatal rat ventricular myocytes (NRVMs) for up to 21 days when cocultured with cardiac fibroblasts, leading to an increase in connexin 43 (Cx43) relative to control cultures (NRVMs cultured on traditional gelatin-coated dishes and RTG hydrogel without AuNPs). This biomimetic and conductive RTG-AuNP hydrogel holds promise for future cardiac tissue engineering applications.