Cytokeratin 8 immunostaining pattern and E-cadherin expression distinguish lobular from ductal breast carcinoma
Cytokeratin 8 immunostaining pattern and E-cadherin expression distinguish lobular from ductal breast carcinoma
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DOI：
10.1309/cpux-kweh-7b26-ye19
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发表时间：
2000-08-01
影响因子：
3.5
通讯作者：
Gown, AM
中科院分区：
文献类型：
--
作者：
Lehr, HA;Folpe, A;Gown, AM
使用细胞角蛋白8抗体的免疫组织化学可以作为鉴别乳腺小叶癌和导管癌的有价值的诊断工具。与导管癌不同，导管癌呈外周为主的免疫染色模式，相邻的肿瘤细胞相互“模压”，小叶癌呈环形核周免疫染色模式，与邻近的肿瘤细胞形成“弹珠袋”的外观。这种免疫染色模式是稳定的，即使在没有表现出特征性组织形态学特征的肿瘤（如小叶癌的实体型或多形型）和模仿其他肿瘤类型特征的肿瘤（如导管癌的靶状或单纵队生长模式）中也是如此。此外，我们发现导管癌以类似的外周显性免疫染色模式表达E-cadherin（33/33例），而所有15例小叶癌均未表达E-cadherin，这表明E-cadherin在肿瘤细胞内细胞骨架支架的结构组织中起作用。
Immunohistochemistry using antibodies to cytokeratin 8 can serve as a valuable diagnostic tool for the differentiation of lobular from ductal carcinomas of the breast. In contrast with ductal carcinomas, which exhibit a peripheral-predominant immunostaining pattern, adjacent tumor cells "molding" to each other lobular carcinomas exhibit a ring-like perinuclear immunostaining pattern, creating a "bag of marbles" appearance with neighboring tumor cells. This immunostaining pattern is stable even in the tumors that otherwise do not exhibit characteristic histomorphologic features (ie, solid or pleomorphic type of a lobular carcinoma) and tumors that mimic growth patterns characteristic of the respective other turner type (ie, targetoid or single-file growth pattern in a ductal carcinoma). Furthermore, we demonstrate that ductal carcinomas express E-cadherin in a similar peripheral-predominant immunostaining pattern (33/33 cases), while all 15 lobular carcinomas were negative for E-cadherin, suggesting a role for E-cadherin in the architectural organization of the cytoskeletal scaffolding within the tumor cells.