Bone marrow lymphoid and myeloid progenitor cells are suppressed in 7,12-dimethylbenz(a)anthracene (DMBA) treated mice.
Bone marrow lymphoid and myeloid progenitor cells are suppressed in 7,12-dimethylbenz(a)anthracene (DMBA) treated mice.
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DOI：
10.1016/j.tox.2010.02.009
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发表时间：
2010-04-30
期刊：
影响因子：
4.5
通讯作者：
Czuprynski, C. J.
中科院分区：
文献类型：
--
作者：
N'jai, A. U.;Larsen, M.;Shi, L.;Jefcoate, C. R.;Czuprynski, C. J.
在这项研究中，我们使用集落形成单位(CFU)试验证明，在DMBA处理的小鼠中，淋巴系(CFU-Preb)和髓系(CFU-GM)祖细胞迅速受到抑制(在6小时内)。这些变化的持续时间与通过ip或口服DMBA获得的DMBA及其代谢物的血液水平一致。CFU-GM和CFU-Preb活性分别在口服DMBA后第2天和第7天恢复到对照水平，但在IP DMBA给药后第7天仍受到抑制。IP给药后血液中持续存在低水平的DMBA与CFU-Preb、总骨髓淋巴样细胞和外周血淋巴细胞的持续抑制有关。在CYP1B1基因缺失的小鼠中未观察到上述变化，表明需要由CYP1B1在骨髓中进行局部DMBA代谢，以损害骨髓CFU-Preb和CFU-GM。此外，这些数据提供了证据表明，在DMBA暴露后，髓系细胞比淋巴系细胞恢复得更快。
In this study we used colony forming unit (CFU) assays to demonstrate rapid suppression (within 6 h) of lymphoid (CFU-preB) and myeloid (CFU-GM) progenitor cells in DMBA treated mice. The duration of these changes were consistent with the blood levels of DMBA and its metabolites that were achieved by either IP or oral DMBA administration. CFU-GM and CFU-preB activities returned to control levels by 2 and 7 days after oral DMBA exposure, respectively, but remained suppressed through 7 days after IP DMBA administration. The continued presence of low levels of DMBA in the bloodstream following IP administration was associated with sustained suppression of CFU-preB, total bone marrow lymphoid cells and peripheral blood lymphocytes. The changes noted above were not observed in Cyp1b1 null mice, demonstrating the need for local DMBA metabolism in the bone marrow by Cyp1b1 to impair bone marrow CFU-preB and CFU-GM. Furthermore, these data provide evidence that myeloid lineage cells are restored more quickly than lymphoid lineage cells after DMBA exposure.
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DOI：
10.1016/0192-0561(86)90058-5
发表时间：
1986-01-01
期刊：
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY
影响因子：
--
作者：
DEAN, JH;WARD, EC;ADAMS, DO
通讯作者：
ADAMS, DO
影响因子：
3.6
作者：
Gao, Jun;Mitchell, Leah A.;Burchiel, Scott W.
通讯作者：
Burchiel, Scott W.
影响因子：
3.8
作者：
Gao, J;Lauer, FT;Burchiel, SW
通讯作者：
Burchiel, SW
影响因子：
3.8
作者：
Galv치n, N;Jaskula-Sztul, R;Jefcoate, CR
通讯作者：
Jefcoate, CR
影响因子：
3.8
作者：
Allan, LL;Mann, KK;Sherr, DH
通讯作者：
Sherr, DH