Multilocus microsatellite typing (MLMT) reveals genetic homogeneity of Leishmania donovani strains in the Indian subcontinent
Multilocus microsatellite typing (MLMT) reveals genetic homogeneity of Leishmania donovani strains in the Indian subcontinent
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DOI：
10.1016/j.meegid.2008.09.005
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Schoenian, Gabriele
中科院分区：
文献类型：
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作者：
Alam, Mohammad Zahangir;Kuhls, Katrin;Schoenian, Gabriele
在对印度次大陆多诺瓦利什曼寄生虫的群体遗传研究中，利用15个高变微卫星位点分析了从孟加拉国、印度、尼泊尔和斯里兰卡的黑热病（100）、黑热病后皮肤利什曼病（PKDL）（25）和皮肤利什曼病（CL）(2)患者和5名临床模式未明确的患者中分离出的132株分离物。采用基于贝叶斯模型的聚类算法，构建基于遗传距离的系统发育树，对多位点微卫星分型（MLMT）数据进行分析。来自孟加拉国、比哈尔邦（印度）和尼泊尔的125株毒株不论其地理来源、临床表现以及对锑类药物的体外或体内敏感性如何，都形成了一个非常同质的种群。108株菌株的多位点微卫星谱图一致。其他菌株只有一个标记不同。值得注意的是，与肯尼亚多诺瓦氏L.亲缘关系最密切的3个印度菌株以及来自印度和斯里兰卡CL病例的4个菌株的微卫星图谱存在显著差异。单一同质种群在比哈尔邦（印度）、孟加拉国和尼泊尔的传播很可能与该地区内脏利什曼病的流行传播有关，(c) 2008 Elsevier B.V.版权所有。
In this population genetic study of Leishmania donovani parasites in the Indian Subcontinent, 132 isolates obtained from patients in Bangladesh, India, Nepal and Sri Lanka suffering from Kala-azar (100), post-Kala-azar dermal leishmaniasis (PKDL) (25) and cutaneous leishmaniasis (CL) (2), and from 5 patients whose clinical patterns were not defined, were analysed by using 15 hyper-variable microsatellite loci. multilocus microsatellite typing (MLMT) data were analysed by using a Bayesian model-based Clustering algorithm and constructing phylogenic tree based on genetic distances. In total, 125 strains from Bangladesh, Bihar (India) and Nepal formed a very homogeneous population regardless of geographical origin, clinical manifestation, and whether they presented in vitro or in Vivo susceptibility to antimonial drugs. Identical multilocus microsatellite profiles were found for 108 strains. other strains differed in only one marker. Considerably, different microsatellite profiles were identified for three Indian strains most closely related to L. donovani from Kenya, and for four strains from Indian and Sri Lankan CL cases. The circulation of a single homogeneous Population of L. donovani in Bihar (India), Bangladesh and Nepal is, most probably, related to the epidemic spread of visceral leishmaniasis in this area, (c) 2008 Elsevier B.V. All rights reserved.