Expression of proton-coupled oligopeptide transporter (POTs) in prostate of mice and patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa)
Expression of proton-coupled oligopeptide transporter (POTs) in prostate of mice and patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa)
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质子偶联寡肽转运蛋白（POT）在小鼠前列腺以及良性前列腺增生（BPH）和前列腺癌（PCa）患者中的表达
DOI：
10.1002/pros.22568
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发表时间：
2013-02-15
期刊：
影响因子：
2.8
通讯作者：
Jiang, Huidi
中科院分区：
文献类型：
--
作者：
Sun, Dongli;Tan, Fuqing;Jiang, Huidi
质子偶联寡肽转运蛋白（POTs）作为细胞摄取二肽/三肽的膜蛋白。前列腺分泌前列腺液需要大量的营养物质。此外，前列腺的药物治疗效果有限。因此，本研究旨在评估POTs在小鼠和人前列腺中的表达，旨在为POTs在前列腺吸收营养素和拟肽药物中的潜在作用提供信息。方法采用实时定量PCR和Western blot方法，检测POTs在前列腺、人前列腺癌细胞（PC-3）和人前列腺上皮细胞（RWPE-1）中的mRNA和蛋白表达。结果qRT-PCR结果显示小鼠前列腺组织中POTs mRNA的表达具有不同的特点。在这些转运蛋白中，检测到PepT 2的蛋白表达，并在小鼠前列腺发育过程中增加，而PepT 1，PHT 1和PHT 2蛋白未检测到。此外，炎症对POTs mRNA表达的调节在RWPE-1和PC-3中具有不同的特点。PCa患者前列腺组织中PepT 2和PHT 1 mRNA表达均低于BPH患者。结论POTs在小鼠前列腺和BPH、PCa患者前列腺中表达，提示POTs可能参与前列腺内二、三肽和肽模拟物的转运。前列腺73：287295，2013年。(c)2012 Wiley Periodicals，Inc.
BACKGROUND Proton-coupled oligopeptide transporters (POTs) serve as integral membrane protein for the cellular uptake of di/tripeptide. Prostate has a large requirement of nutriment for its function to produce and secrete prostatic fluid. Besides, prostate suffered from limited therapy effect of drug treatment. Thus present study was performed to evaluate the expression of POTs in prostate of mice and human with the aim to provide information for potential role of POTs in absorption of nutriment and peptidomimetic drugs in prostate. METHODS Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot methods were applied to study the mRNA, protein expression of POTs in prostate, human prostate cancer cells (PC-3), and human prostate epithelial cells (RWPE-1). RESULTS qRT-PCR study showed different characteristic of POTs mRNA expression in mouse prostate. Among these transporters, protein expression of PepT2 was detected and increasing during the development of mouse prostate, while PepT1, PHT1, and PHT2 protein was not detected. Furthermore, different characteristic of regulation by inflammation on POTs mRNA expression was found in RWPE-1 and PC-3. In addition, mRNA expression of PepT2 and PHT1 in prostate of patients with PCa was demonstrated be lower compared with BPH. CONCLUSIONS These findings provide the first evidence for the expression of POTs in prostate of mice and patients with BPH or PCa and suggest that POTs are likely to play a role in the transport of di/tripeptides and peptidomimetics in prostate. Prostate 73: 287295, 2013. (c) 2012 Wiley Periodicals, Inc.