Cathepsin L occupies a vacuolar compartment and is a protein maturase within the endo/exocytic system of Toxoplasma gondii.
Cathepsin L occupies a vacuolar compartment and is a protein maturase within the endo/exocytic system of Toxoplasma gondii.
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DOI：
10.1111/j.1365-2958.2010.07181.x
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发表时间：
2010-06
影响因子：
3.6
通讯作者：
Carruthers VB
中科院分区：
文献类型：
--
作者：
Parussini F;Coppens I;Shah PP;Diamond SL;Carruthers VB
受调节的胞吐作用允许蛋白质和其他大分子在需要时精确地及时递送以实现其功能。细胞内寄生虫弓形虫在所有单细胞生物中具有最广泛的调节外泌系统之一，但对该系统中蛋白质运输和蛋白水解修饰的基础知之甚少。我们证明，寄生虫的组织蛋白酶蛋白酶，TgCPL，占据了一个新认识的液泡室（VAC），经历动态片段化期间T。弓形虫复制我们还提供的证据表明，在VAC或晚期内体，这种蛋白酶介导的蛋白水解成熟的前体蛋白靶向微线，调节分泌细胞器，提供粘附蛋白的寄生虫表面在细胞入侵。我们的研究结果表明，微线体前体的加工发生在胞吐系统内的中间内吞隔室内，表明在这种人类寄生虫的内吞和胞吐途径的广泛收敛。
Regulated exocytosis allows the timely delivery of proteins and other macromolecules precisely when they are needed to fulfill their functions. The intracellular parasite Toxoplasma gondii has one of the most extensive regulated exocytic systems among all unicellular organisms, yet the basis of protein trafficking and proteolytic modification in this system is poorly understood. We demonstrate that a parasite cathepsin protease, TgCPL, occupies a newly recognized vacuolar compartment (VAC) that undergoes dynamic fragmentation during T. gondii replication. We also provide evidence that within the VAC or late endosome this protease mediates the proteolytic maturation of proproteins targeted to micronemes, regulated secretory organelles that deliver adhesive proteins to the parasite surface during cell invasion. Our findings suggest that processing of microneme precursors occurs within intermediate endocytic compartments within the exocytic system, indicating an extensive convergence of the endocytic and exocytic pathways in this human parasite.
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影响因子：
1.5
作者：
Huang, Robert;Que, Xuchu;Hirata, Ken;Brinen, Linda S.;Lee, Ji Hyun;Hansell, Elizabeth;Engel, Juan;Sajid, Mohammed;Reed, Sharon
通讯作者：
Reed, Sharon
影响因子：
2.1
作者：
Botero-Kleiven, S;Fernández, V;Wahlgren, M
通讯作者：
Wahlgren, M
微生物蛋白在弓形虫贡献毒力中的协同作用。
影响因子：
15.3
作者：
Céréde, O;Dubremetz, JF;Soête, M;Deslée, D;Vial, H;Bout, D;Lebrun, M
通讯作者：
Lebrun, M
DOI：
10.1083/jcb.200612131
发表时间：
2007-07-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Manderson AP;Kay JG;Hammond LA;Brown DL;Stow JL
通讯作者：
Stow JL
影响因子：
--
作者：
El Hajj, Hiba;Papoin, Julien;Lebrun, Maryse
通讯作者：
Lebrun, Maryse