Ultrastructural and molecular characterization of cyanobacterial symbionts in Dictyocoryne profunda (polycystine radiolaria)
Ultrastructural and molecular characterization of cyanobacterial symbionts in Dictyocoryne profunda (polycystine radiolaria)
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DOI：
10.1007/s13199-012-0174-2
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发表时间：
2012-05
期刊：
影响因子：
2.5
通讯作者：
Tomoko Yuasa;T. Horiguchi;S. Mayama;A. Matsuoka;O. Takahashi
中科院分区：
文献类型：
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作者：
Tomoko Yuasa;T. Horiguchi;S. Mayama;A. Matsuoka;O. Takahashi
在多胱氨酸放射虫Dictyocoryne profundaEscherberg的细胞质外发现了蓝藻共生体。透射电镜下观察到细菌共生体呈球形，直径约0.5-1.0 μm。它们存在于一个非常有限的位置，靠近宿主放射虫壳的外围，邻近中央囊壁。它们中的一些细胞可能已经处于细胞分裂过程中或刚刚分裂。共生体具有类囊体结构，以两层或三层同心的方式围绕细胞外围。基于小亚基核糖体DNA（16 S rDNA）系统发育分析，胞内共生细菌与蓝细菌聚球藻（Synechococcus）属的蓝藻聚球藻（Synechococcus）聚球藻（Synechococcus）聚球藻（Synechococcus）聚球藻（Synechococcus）。三个序列，每个来自三个标本的D。2009年3月/10月和2010年3月从东海采集的深海集球藻（Synechococcusclade II）与聚球藻属（Synechococcusclade II）相同，属于聚球藻属（Synechococcusclade II）分支，其特征是藻胆素（phycourobilin，PUB）含量低。
Cyanobacterial symbionts were detected in the extracytoplasm of the polycystine radiolarianDictyocoryne profundaEhrenberg. The bacterial symbionts were observed as numerous spherical bodies ~0.5–1.0 μm in diameter under transmission electron microscopy. They were present in a very restricted location close to the periphery of the host radiolarian shell, adjacent to the central capsular wall. Several cells of them may have been in the process of cell division or just divided. The symbionts had thylakoid-like structures, which ran around the cell periphery in two or three concentric layers. Based on the small subunit ribosomal DNA (16S rDNA) phylogenetic analyses, the intracellular symbiotic bacteria grouped with cyanobacteria belonging to the genusSynechococcus. Three sequences, one from each of three specimens ofD. profunda, collected in March/October 2009 and March 2010 from the East China Sea, were the same and branched withinSynechococcusclade II, that is characterized by strains with low amounts of phycourobilin (PUB).