Determination of miRNA targets in skeletal muscle cells.
Determination of miRNA targets in skeletal muscle cells.
复制标题
DOI：
10.1007/978-1-61779-343-1_28
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Wang, Da-Zhi
中科院分区：
文献类型：
--
作者：
Huang, Zhan-Peng;Espinoza-Lewis, Ramon;Wang, Da-Zhi
microRNAs（miRNAs）是一类由约22个核苷酸组成的非编码小RNA，通过破坏靶mRNA的稳定性从而降解靶mRNA或抑制靶mRNA的翻译，在转录后水平调控基因表达。越来越多的证据表明，miRNAs对哺乳动物的正常发育、稳态和许多其他功能至关重要。此外，miRNA表达的有害变化与人类疾病有关。几种肌肉特异性miRNA，包括miR-1，miR-133，miR-206和miR-208，已被证明对正常的肌母细胞分化，增殖和肌肉重塑响应压力是重要的。它们也与各种心脏和骨骼肌疾病有关。基于miRNA的基因疗法在心脏和骨骼肌疾病的治疗中具有巨大的潜力。在此，我们描述了通常用于研究miRNA的生物学作用的方法，以及用于操纵miRNA表达和研究其靶调控的技术。
MicroRNAs (miRNAs) are a class of small ∼22 nucleotide noncoding RNAs which regulate gene expression at the posttranscriptional level by either destabilizing and consequently degrading their targeted mRNAs or by repressing their translation. Emerging evidence has demonstrated that miRNAs are essential for normal mammalian development, homeostasis, and many other functions. In addition, deleterious changes in miRNA expression were associated with human diseases. Several muscle-specific miRNAs, including miR-1, miR-133, miR-206, and miR-208, have been shown to be important for normal myo-blast differentiation, proliferation, and muscle remodeling in response to stress. They have also been implicated in various cardiac and skeletal muscular diseases. miRNA-based gene therapies hold great potential for the treatment of cardiac and skeletal muscle diseases. Herein, we describe methods commonly applied to study the biological role of miRNAs, as well as techniques utilized to manipulate miRNA expression and to investigate their target regulation.