Enhanced Phenol Biodegradation and Aerobic Granulation by Two Coaggregating Bacterial Strains
Enhanced Phenol Biodegradation and Aerobic Granulation by Two Coaggregating Bacterial Strains
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DOI：
10.1021/es0609295.s001
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H. E-L O N G J I A N G-H.-E-L-O-N-G-J-I-A-N-G-2248938950;J. O O-H W A T A Y-J.-O-O-H-W-A-T-A-Y-2097435079;S. T E P H E N T I O N G-L E E T A Y-S.-T-E-P-H-E-N-T-I-O-N-G-L-E-E-T-A-Y-2106176415
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H. E-L O N G J I A N G-H.-E-L-O-N-G-J-I-A-N-G-2248938950;J. O O-H W A T A Y-J.-O-O-H-W-A-T-A-Y-2097435079;S. T E P H E N T I O N G-L E E T A Y-S.-T-E-P-H-E-N-T-I-O-N-G-L-E-E-T-A-Y-2106176415
两种细菌菌株 Propioniferax-like PG-02 和 Comamonas sp 的共聚集效果。研究了PG-08对苯酚降解和好氧造粒的影响。 PG-02 是一种半饱和动力学常数较低的苯酚降解剂，而 PG-08 具有较强的聚集能力，但苯酚降解能力较差。两种菌株通过凝集素与菌株 PG-02 上的粘附素蛋白和菌株 PG-08 上的互补糖受体的相互作用而共聚集。使用哈维氏弧菌报告菌株 BB170，发现这两种菌株都能产生自诱导剂 2 样信号。如果一起孵育，这两种菌株会协同降解苯酚。在批次中，共培养物以 250 mg L-1 的初始浓度降解苯酚，比每个菌株单独降解的速度更快。与单一培养生物强化相比，在序批式反应器中同时使用两种菌株的生物强化显着改善了苯酚去除和好氧造粒。细菌共聚集可能是好氧造粒过程的一个组成部分。研究好氧造粒中共聚集的原位发生将有助于揭示其分子机制。然后可以通过选择特定的微生物群来改进造粒过程。
The effect of coaggregation of the two bacterial strains Propioniferax-like PG-02 and Comamonas sp. PG-08 on phenol degradation and aerobic granulation was investigated. While PG-02 was characterized as a phenol-degrader with a low half-saturation kinetics constant, PG-08 possessed strong aggregation ability with poor phenol degradation ability. The two strains coaggregated through involvement of lectinsaccharide interactions with the adhesin protein on strain PG-02 and the complementary sugar receptor on strain PG-08. Using the V. harveyi reporter strain BB170, it was found that both strains could produce autoinducer-2like signals. If incubated together, the two strains showed cooperation for phenol degradation. In batch, the coculture degraded phenol at an initial concentration of 250 mg L-1, faster than each strain separately. Bioaugmentation with simultaneously the two strains in sequencing batch reactors significantly improved phenol removal and aerobic granulation as compared to monoculture bioaugmentation. Bacterial coaggregation might be an integral component of the aerobic granulation process. Investigation of in situ occurrence of coggregation in aerobic granulation would help unveil its molecular mechanism. Then the granulation process could be improved through selection of specific microbial groups.