Cis-Golgi cisternal assembly and biosynthetic activation occur sequentially in plants and algae.
Cis-Golgi cisternal assembly and biosynthetic activation occur sequentially in plants and algae.
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DOI：
10.1111/tra.12052
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Staehelin LA
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文献类型：
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作者：
Donohoe BS;Kang BH;Gerl MJ;Gergely ZR;McMichael CM;Bednarek SY;Staehelin LA
高尔基体运输的脑池进展/成熟模型预测，顺式高尔基体脑池在高尔基体的顺式侧从头形成。在这里，我们描述的顺式池组装过程中的高压冷冻藻类（Scherffelia dubia，衣藻reinhardtii）和植物（拟南芥，Dionaea muscipula;捕蝇草）的结构和功能的中间体，通过电子显微镜，电子断层扫描和免疫电子显微镜技术确定。我们的调查结果如下：最顺式（C1）高尔基体池由3-5个COPII囊泡与C2顺式池接触融合产生的池引发剂从头产生。COPII囊泡为引发剂的生长提供燃料，然后引发剂合并成连贯的C1池。当一个C1池成核它的第一个池引发剂，它成为一个C2池。C2-Cn顺式池通过COPII囊泡融合生长。ER驻留蛋白通过COPIa型囊泡从顺式脑池再循环到ER。In S. dubia的C2池能够介导规模蛋白复合物的自组装。在植物中，约90%的天然α-甘露糖苷酶I定位于内侧高尔基池。顺式脑池的生物化学活化似乎与它们通过中间脑池酶的再循环转化为中间脑池相一致。我们提出植物和藻类的不同顺式池组装中间体实际上可能如何与哺乳动物细胞中ERGIC和顺式前高尔基池层中存在的中间体相关。
The cisternal progression/maturation model of Golgi trafficking predicts that cis-Golgi cisternae are formed de novo on the cis-side of the Golgi. Here we describe structural and functional intermediates of the cis cisterna assembly process in high-pressure frozen algae (Scherffelia dubia, Chlamydomonas reinhardtii) and plants (Arabidopsis thaliana, Dionaea muscipula; Venus Flytrap) as determined by electron microscopy, electron tomography and immuno-electron microscopy techniques. Our findings are as follows: The cis-most (C1) Golgi cisternae are generated de novo from cisterna initiators produced by the fusion of 3–5 COPII vesicles in contact with a C2 cis cisterna. COPII vesicles fuel the growth of the initiators, which then merge into a coherent C1 cisterna. When a C1 cisterna nucleates its first cisterna initiator it becomes a C2 cisterna. C2-Cn cis cisternae grow through COPII vesicle fusion. ER-resident proteins are recycled from cis cisternae to the ER via COPIa-type vesicles. In S. dubia the C2 cisternae are capable of mediating the self-assembly of scale protein complexes. In plants, ~90% of native α-mannosidase I localizes to medial Golgi cisternae. Biochemical activation of cis cisternae appears to coincide with their conversion to medial cisternae via recycling of medial cisterna enzymes. We propose how the different cis cisterna assembly intermediates of plants and algae may actually be related to those present in the ERGIC and in the pre-cis Golgi cisterna layer in mammalian cells.