Discrimination of closely homologous HPV types by nonisotopicin situ hybridization: definition and derivation of tissue melting temperatures
Discrimination of closely homologous HPV types by nonisotopicin situ hybridization: definition and derivation of tissue melting temperatures
复制标题
通过非同位素原位杂交区分密切同源的 HPV 类型：组织熔化温度的定义和推导
DOI：
10.1007/bf01005977
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
J. McGee
中科院分区：
文献类型：
--
作者：
C. Herrington;A. Graham;D. Flannery;J. Burns;J. McGee
SummaryIt is generally assumed that nucleic acid association duringin situ hybridization reactions is similar to that of nucleic acid association in solution. This assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 byin situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies.By examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical DNA-DNA stability curves and their derivative equations for tissue melting temperatures (Tmt) were derived. The corresponding values for Tmt are 10–20°C higher than their solution equivalents. These data, supported by polymerase chain reaction experiments, demonstrate that closely homologous viral DNAs cross linked in tissue by formaldehyde fixation do not interact with the corresponding labelled probes as predicted from solution kinetic equations. This not only has theoretical implications but is also relevant to the accuracy of clinical diagnostic testing.
人乳头瘤病毒 (HPV) 感染的生物学及其在鳞状细胞癌发生中的作用。
DOI：
--
发表时间：
1987
期刊：
Medical biology
影响因子：
--
作者：
Syrjänen,KJ
通讯作者：
Syrjänen,KJ
原位杂交鉴定男性尿道尖锐湿疣人乳头瘤病毒类型。
影响因子：
3.3
作者：
DelMistro,A;Braunstein,JD;Halwer,M;Koss,LG
通讯作者：
Koss,LG
通过原位杂交表征儿童尖锐湿疣中的人乳头瘤病毒类型。
DOI：
--
发表时间：
1987
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Vallejos,H;DelMistro,A;Kleinhaus,S;Braunstein,JD;Halwer,M;Koss,LG
通讯作者：
Koss,LG