Targeted 19F-tags to detect amino acids in complex mixtures using NMR spectroscopy
Targeted 19F-tags to detect amino acids in complex mixtures using NMR spectroscopy
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DOI：
10.1016/j.jfluchem.2022.110084
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发表时间：
2023-01-05
影响因子：
1.9
通讯作者：
Krishnan，V. V.
中科院分区：
文献类型：
--
作者：
Montgomery，Keeton;Elhabashy，Aya;Krishnan，V. V.
氟-19核的核磁共振光谱（19 F-NMR）由于氟-19的高天然丰度、宽光谱范围和自旋半系统的简单性而成为强有力的工具。然而，它在化学课堂教学和科研中的应用还很少。因此，本文的目的是证明NMR的力量，通过调查的动力学时，a19 F-标签与个别氨基酸（AA）反应，并最终利用该方法来识别和定量各种氨基酸从一个复杂的混合物，如代谢组学样品。按照先前建立的方法合成了命名为2，5-二氧代吡咯烷-1-基-2-（三氟甲基）苯甲酸酯的19 F-标签。然后在选定的AA存在下，使用19 F NMR连续测量标签的反应动力学。估计的反应速率常数，以形成19 F-标签与每个AA不同，这可以被用作一个识别工具。对于所有样品，标签形成通常在水中在24-48小时内完成。这些证明表明，19 F-tags可以形成化学动力学和AA检测使用19 F-NMR的基础。
Nuclear magnetic resonance spectroscopy of fluorine-19 nucleus (19F-NMR) emerges as a powerful tool because of the high sensitivity due to the high natural abundance of fluorine-19, broad spectral range, and the simplicity of a spin-half system. However, it is still seldom utilized in the chemistry classroom or research. This article thus aims to demonstrate the power of NMR by investigating the kinetics when a19F-tag reacts with individual amino acids (AA) and eventually utilizing the approach to identify and quantify various AAs from a complex mixture such as a metabolomics sample. The19F-tag named 2,5-dioxopyrrolidin-1-yl-2-(trifluoromethyl)benzoate was synthesized following a previously established method. The reaction kinetics of the tag was then continuously measured using19F NMR in the presence of selected AAs. The estimated reaction rate constants to form the19F-tags with each AA differ, which could be used as an identification tool. The tag formations were typically completed in 24-48 hours in water for all the samples. These demonstrations suggest that19F-tags could form the basis for chemical kinetics and AA detection using19F-NMR.