An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica - Fritillaria usuriensis dropping pills for pulmonary diseases.
An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica - Fritillaria usuriensis dropping pills for pulmonary diseases.
复制标题
综合化学组学和系统生物学方法分析中药制剂枇杷贝母滴丸治疗肺部疾病的作用机制
DOI：
10.1186/s12906-015-0983-y
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发表时间：
2016-01-08
影响因子：
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通讯作者：
Bai G
中科院分区：
文献类型：
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作者：
Tao J;Hou Y;Ma X;Liu D;Tong Y;Zhou H;Gao J;Bai G
传统中药 (TCM) 草药配方提供了有价值的治疗策略。然而，大多数这些配方的活性成分和作用机制仍不清楚。因此，复杂机制的识别是中医研究的重大挑战。本研究采用网络药理学方法阐明中药制剂枇杷-贝母滴丸（川贝琵琶滴丸，CBPP）的抗炎、止咳机制。通过高质量超高效液相色谱/四极杆飞行时间质谱（UPLC/Q-TOF-MS）鉴定CBPP的化学成分，并使用PharmMapper和京都基因与基因组百科全书（KEGG）生物信息学网站选择和分析抗炎成分，分别预测目标蛋白和相关通路。然后，进行RNA测序（RNA-Seq）分析来研究慢性支气管炎大鼠肺组织中基因的不同表达。六种主要成分影响 19 条预测途径，包括来自 Eriobotrya japonica (Thunb.) Lindl 的熊果酸和齐墩果酸。 (Eri)，贝母碱，来自乌苏里贝母 Maxim。 （星期五），来自桔梗 (Jacq.) A. DC 的桔梗皂甙元和聚半乳酸。 (Pla) 和来自半夏 (Thunb.) Makino 的鸟苷。 （别针）。 CBPP治疗后34个基因的表达显着下降，影响免疫调节、抗炎、胶原形成和肌肉收缩四种治疗功能。活性成分作用于丝裂原激活蛋白激酶（MAPK）通路、转化生长因子（TGF）-β通路、粘着斑、紧密连接及细胞骨架，发挥抗炎、化痰、止咳作用。这种全球化学组学整合系统生物学的新方法为多成分、多靶点机制的中药研究提供了一种有效而准确的策略。本文的在线版本 (doi:10.1186/s12906-015-0983-y) 包含补充材料，可供授权用户使用。
Traditional Chinese medicine (TCM) herbal formulae provide valuable therapeutic strategies. However, the active ingredients and mechanisms of action remain unclear for most of these formulae. Therefore, the identification of complex mechanisms is a major challenge in TCM research. This study used a network pharmacology approach to clarify the anti-inflammatory and cough suppressing mechanisms of the Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills (ChuanbeiPipa dropping pills, CBPP). The chemical constituents of CBPP were identified by high-quality ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), and anti-inflammatory ingredients were selected and analyzed using the PharmMapper and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatics websites to predict the target proteins and related pathways, respectively. Then, an RNA-sequencing (RNA-Seq) analysis was carried out to investigate the different expression of genes in the lung tissue of rats with chronic bronchitis. Six main constituents affected 19 predicted pathways, including ursolic acid and oleanolic acid from Eriobotrya japonica (Thunb.) Lindl. (Eri), peiminine from Fritillaria usuriensis Maxim. (Fri), platycodigenin and polygalacic acid from Platycodon grandiflorum (Jacq.) A. DC. (Pla) and guanosine from Pinellia ternata (Thunb.) Makino. (Pin). Expression of 34 genes was significantly decreased after CBPP treatment, affecting four therapeutic functions: immunoregulation, anti-inflammation, collagen formation and muscle contraction. The active components acted on the mitogen activated protein kinase (MAPK) pathway, transforming growth factor (TGF)-beta pathway, focal adhesion, tight junctions and the action cytoskeleton to exert anti-inflammatory effects, resolve phlegm, and relieve cough. This novel approach of global chemomics-integrated systems biology represents an effective and accurate strategy for the study of TCM with multiple components and multiple target mechanisms. The online version of this article (doi:10.1186/s12906-015-0983-y) contains supplementary material, which is available to authorized users.