A novel role for protein-tyrosine kinase Etk from Escherichia coli K-12 related to polymyxin resistance
A novel role for protein-tyrosine kinase Etk from Escherichia coli K-12 related to polymyxin resistance
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DOI：
10.1016/j.resmic.2006.01.003
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发表时间：
2006-09-01
影响因子：
2.6
通讯作者：
Grangeasse, Christophe
中科院分区：
文献类型：
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作者：
Lacour, Soline;Doublet, Patricia;Grangeasse, Christophe
蛋白酪氨酸激酶在细菌多粘菌素耐药中的作用通过遗传和生化方法进行了评估。通过一步灭活染色体基因的方法，分别敲除了编码蛋白酪氨酸激酶的两个基因wzc和etk，并通过测量存活细胞的百分比来检测相应的突变株对不同浓度的多粘菌素B的抗性。对双敲除wzc-ETK突变体的抗性进行了分析，并通过检测表达wzc和elk的质粒载体的效果进行了互补实验。我们的结果一致地表明，蛋白激酶WZC在多粘菌素耐药性中不是必不可少的，而蛋白激酶ETK似乎在这种抗生素耐药性中起着关键作用。这一新发现的ETK的特定功能强化了蛋白质-酪氨酸激酶参与细菌生理的不同方面的概念。(C)2006年爱思唯尔公司。版权所有。
The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the corresponding mutant strain was assayed in each case for resistance to different concentrations of polymyxin B by measuring the percentage of surviving cells. The resistance of a double knock-out wzc-etk-mutant was also analyzed and complementation experiments were performed by checking the effect of plasmid vectors expressing either Wzc or Elk. Our results concurred in showing that protein-kinase Wzc is not essential for polymyxin resistance, whereas protem-kinase Etk appears to play a key role in such antibiotic resistance. This newly found specific function of Etk reinforces the concept that protein-tyrosine kinases are involved in distinct facets of bacterial physiology. (c) 2006 Elsevier SAS. All rights reserved.