Multilocus sequence typing of 102 Burkholderia pseudomallei strains isolated from China
Multilocus sequence typing of 102 Burkholderia pseudomallei strains isolated from China
复制标题
102株中国分离的鼻疽伯克霍尔德菌的多位点序列分型
DOI：
10.1017/s0950268815003052
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发表时间：
2016-07-01
影响因子：
4.2
通讯作者：
Mao, X.
中科院分区：
文献类型：
--
作者：
Fang, Y.;Zhu, P.;Mao, X.
应用多位点序列分型技术对中国地区不同地理和种群来源的102株假鼻疽伯克霍尔德氏菌的系统发育和流行病学关系进行了研究。使用e-Burst算法对MLST数据进行分析，并根据菌株等位基因图谱的成对差异构建了算术平均树形图的未加权配对组法。共鉴定出41个序列类型，其中8个为新序列(ST1341、ST1345、ST1346、ST1347、ST1348、ST1349、ST1350、ST1351)。没有确定特定的地理或宿主种群的系统发育谱系。ST46、ST50、ST55、ST58、ST70和ST1095是优势菌株，但约44%的菌株被划分为45个STS，表明该菌株具有较高的遗传多样性。此外，中国的优势STS与泰国的假鼻疽菌分离株具有显著的连锁关系。对gmhD等位基因的分析表明，中国假鼻疽菌具有较高的遗传变异。
SUMMARY The phylogenetic and epidemiological relationships of 102 Burkholderia pseudomallei clinical isolates from different geographical and population sources in China were investigated by multilocus sequence typing (MLST). The MLST data were analysed using the e-BURST algorithm, and an unweighted pair-group method with arithmetic mean dendrogram was constructed based on the pair-wise differences in the allelic profiles of the strains. Forty-one sequence types (STs) were identified, of which eight were novel (ST1341, ST1345, ST1346, ST1347, ST1348, ST1349, ST1350, ST1351). No geographical-specific or host population-specific phylogenetic lineages were identified. ST46, ST50, ST55, ST58, ST70 and ST1095 predominated, but ~44% of isolates were assigned to 45 STs illustrating high genetic diversity in the strain collection. Additionally, the phylogenetic relationships of the dominant STs in China showed significant linkeage with B. pseudomallei isolates from Thailand. Analysis of the gmhD allele suggests high genetic variation in B. pseudomallei in China.