Analysis of Staphylococcus aureus cell viability, sublethal injury and death induced by synergistic combination of ultrasound and mild heat
Analysis of Staphylococcus aureus cell viability, sublethal injury and death induced by synergistic combination of ultrasound and mild heat
复制标题
超声与微热协同组合诱导金黄色葡萄球菌细胞活力、亚致死损伤和死亡分析
DOI：
10.1016/j.ultsonch.2017.04.019
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发表时间：
2017-11-01
影响因子：
8.4
通讯作者：
Ding, Tian
中科院分区：
文献类型：
--
作者：
Li, Jiao;Suo, Yuanjie;Ding, Tian
本研究旨在探讨超声和温和加热对S.金黄色葡萄球菌与细胞膜完整性和细胞内酶活性相关。在55 ℃下超声处理细胞3、5、7、10和15 min。使用流式细胞术测定来评价金黄色葡萄球菌细胞活力、亚致死损伤和死亡。显微镜下观察了S.金黄色。结果表明，超声波处理过程中，细胞膜损伤与酯酶抑制同步发生，导致细胞立即死亡。另一方面，在55摄氏度的温和加热下的细菌通过亚致死损伤过程被灭活。结果表明，超声波处理和温热处理对黄瓜枯萎病菌的致死方式不同，二者具有协同作用。金黄色。热超声处理的抑菌值大于单独处理的总和。热超声处理组合协同减少亚致死细胞的数量，也导致严重的细胞损伤。
This study was designed to investigate the combined effects of ultrasound and mild heat on the viability of S. aureus in association with the cell membrane integrity and intracellular enzyme activity. Cells were treated by ultrasound under 55 degrees C for 3, 5, 7, 10, and 15 min. The dynamic changes of S. aureus cell viability, sublethal injury and death were evaluated using flow cytometric assay. Microscopies were applied to identify the morphological appearance, ultrastructure and topography of S. aureus. The results showed the membrane damage was synchronous with esterase inhibition during the exposure to sonication, leading to the immediate lethal effect. On the other hand, bacteria under the mild heat at 55 degrees C were inactivated via a sublethal injury process. The different lethal modes were observed between sonication and mild heat treatments, which could synergistically inactivate S. aureus. The antibacterial value of thermo-sonication was greater than the sum of the individual treatments. The thermo-sonication combination synergistically reduced the number of sublethal cells and also resulted in severe cell damage.