Triplex DNA Helix Sensor Based on Reduced Graphene Oxide and Electrodeposited Gold Nanoparticles for Sensitive Lead(II) Detection.
Triplex DNA Helix Sensor Based on Reduced Graphene Oxide and Electrodeposited Gold Nanoparticles for Sensitive Lead(II) Detection.
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DOI：
10.3390/toxics11090795
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发表时间：
2023-09-20
期刊：
影响因子：
4.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Gao J;Xu P;Qiao L;Tao Y;Xiao Y;Qin H;Zhu Y;Zhang Y
基于还原氧化石墨烯（rGO）和电沉积金纳米粒子（EAu）构建了一种三链DNA电化学传感器，用于Pb 2+的检测。在玻碳电极（GCE）上依次电沉积rGO和EAu，再修饰一个由富含鸟嘌呤（G）的环和以T-A·T碱基三联体为基础的三螺旋主干组成的三螺旋DNA。在Pb ~（2+）存在下，由Watson-Crick和Hoogsteen碱基配对形成的DNA构型发生断裂，转化为G-四链体。信号指示剂亚甲基蓝（MB）提供了足够的电化学响应信号。该传感器在0.01 ~ 100.00 μM范围内，Pb 2+浓度的对数与微分脉冲伏安（DPV）峰电流呈线性关系，检测限为0.36 nM。该传感器还与自来水，河流和医疗废水样品进行了测试，合格的回收率和准确性，并代表了一个有前途的方法检测Pb 2+。
A triplex DNA electrochemical sensor based on reduced graphene oxide (rGO) and electrodeposited gold nanoparticles (EAu) was simply fabricated for Pb2+ detection. The glass carbon electrode (GCE) sequentially electrodeposited with rGO and EAu was further modified with a triplex DNA helix that consisted of a guanine (G)-rich circle and a stem of triplex helix based on T-A•T base triplets. With the existence of Pb2+, the DNA configuration which was formed via the Watson–Crick and Hoogsteen base pairings was split and transformed into a G-quadruplex. An adequate electrochemical response signal was provided by the signal indicator methylene blue (MB). The proposed sensor demonstrated a linear relationship between the differential pulse voltammetry (DPV) peak currents and the logarithm of Pb2+ concentrations from 0.01 to 100.00 μM with a detection limit of 0.36 nM. The proposed sensor was also tested with tap water, river and medical wastewater samples with qualified recovery and accuracy and represented a promising method for Pb2+ detection.