Lectin Fingerprinting Distinguishes Antibody Neutralization in SARS-CoV-2.
Lectin Fingerprinting Distinguishes Antibody Neutralization in SARS-CoV-2.
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DOI：
10.1021/acscentsci.2c01471
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发表时间：
2023-05-24
影响因子：
18.2
通讯作者：
Kiessling, Laura L.
中科院分区：
文献类型：
--
作者：
Wuo, Michael G.;Dugan, Amanda E.;Halim, Melanie;Hauser, Blake M.;Feldman, Jared;Caradonna, Timothy M.;Zhang, Shuting;Pepi, Lauren E.;Atyeo, Caroline;Fischinger, Stephanie;Alter, Galit;Garcia-Beltran, Wilfredo F.;Azadi, Parastoo;Hung, Deb;Schmidt, Aaron G.;Kiessling, Laura L.
包膜病毒利用宿主糖基化途径修饰其表面蛋白。随着病毒的进化，新出现的病毒株可以改变其糖基化模式，以影响宿主相互作用并破坏免疫识别。尽管如此，病毒糖基化的变化或其对抗体保护的影响不能单独从基因组序列预测。使用高度糖基化的SARS-CoV-2刺突蛋白作为模型系统，我们提出了一种凝集素指纹分析方法，该方法快速报告与抗体中和相关的变体糖基化状态的变化。在存在抗体或恢复期和接种疫苗的患者血清的情况下，出现独特的凝集素指纹，其区分中和抗体与非中和抗体。该信息不能仅从抗体与刺突受体结合结构域（RBD）结合数据之间的直接结合相互作用推断。野生型（Wuhan-Hu-1）和Delta（B.1.617.2）变体的刺突RBD的比较糖蛋白质组学显示O-糖基化差异是免疫识别差异的关键决定因素。这些数据强调了病毒糖基化和免疫识别之间的相互作用，并揭示了凝集素指纹图谱是一种快速、灵敏和高通量的测定方法，可用于区分靶向关键病毒糖蛋白的抗体的中和潜力。凝集素指纹分析是一种高通量和简便的方法，可以快速分析针对病毒糖蛋白产生的患者抗体的中和潜力。
Enveloped viruses co-opt host glycosylation pathways to decorate their surface proteins. As viruses evolve, emerging strains can modify their glycosylation patterns to influence host interactions and subvert immune recognition. Still, changes in viral glycosylation or their impact on antibody protection cannot be predicted from genomic sequences alone. Using the highly glycosylated SARS-CoV-2 Spike protein as a model system, we present a lectin fingerprinting method that rapidly reports on changes in variant glycosylation state, which are linked to antibody neutralization. In the presence of antibodies or convalescent and vaccinated patient sera, unique lectin fingerprints emerge that distinguish neutralizing versus non-neutralizing antibodies. This information could not be inferred from direct binding interactions between antibodies and the Spike receptor-binding domain (RBD) binding data alone. Comparative glycoproteomics of the Spike RBD of wild-type (Wuhan-Hu-1) and Delta (B.1.617.2) variants reveal O-glycosylation differences as a key determinant of immune recognition differences. These data underscore the interplay between viral glycosylation and immune recognition and reveal lectin fingerprinting to be a rapid, sensitive, and high-throughput assay to distinguish the neutralization potential of antibodies that target critical viral glycoproteins. Lectin fingerprinting is a high-throughput and facile method to rapidly profile the neutralization potential of patient antibodies raised against viral glycoproteins.
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影响因子：
4
作者：
Bojar, Daniel;Meche, Lawrence;Meng, Guanmin;Eng, William;Smith, David F.;Cummings, Richard D.;Mahal, Lara K.
通讯作者：
Mahal, Lara K.
DOI：
10.1126/science.abl6251
发表时间：
2022-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Byrnes, James R.;Zhou, Xin X.;Wells, James A.
通讯作者：
Wells, James A.
病毒进入糖蛋白的秘密生活：免疫逃避中的月光。
影响因子：
6.7
作者：
Cook JD;Lee JE
通讯作者：
Lee JE
影响因子：
64.5
作者：
Kang L;He G;Sharp AK;Wang X;Brown AM;Michalak P;Weger-Lucarelli J
通讯作者：
Weger-Lucarelli J