KINETICS OF REACTION OF N-ETHYLMALEIMIDE WITH DENATURED BETA-LACTOGLOBULIN AND OVALBUMIN
KINETICS OF REACTION OF N-ETHYLMALEIMIDE WITH DENATURED BETA-LACTOGLOBULIN AND OVALBUMIN
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DOI：
10.1016/0005-2795(68)90206-7
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发表时间：
1968-01-01
期刊：
影响因子：
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通讯作者：
LESLIE, J
中科院分区：
文献类型：
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作者：
FRANKLIN, JG;LESLIE, J
[β]-乳球蛋白与N-乙基马来酰亚胺的反应动力学进行了研究，以将巯基的反应性与尿素和十二烷基硫酸钠溶液中蛋白质的结构联系起来。在8 M尿素中的反应是2级，但在十二烷基硫酸钠中观察到与2级动力学的微小偏差。在8 M尿素中的二阶速率常数与半胱氨酸的相同反应相似，但[β]-乳球蛋白与N-乙基马来酰亚胺在十二烷基硫酸钠中的反应速率比在8 M尿素中慢约150倍。蛋白质的光谱性质的变化发生在与N-乙基马来酰亚胺或氯化汞在一定条件下的尿素浓度和pH值的反应。卵清蛋白与N-乙基马来酰亚胺在十二烷基硫酸钠的反应不遵循简单的二阶动力学。
The kinetics of the reaction of [beta]-lacto-globulin with N-ethylmaleimide were studied in order to relate the reactivity of the sulfhydryl groups to the structure of the protein in urea and sodium dodecyl sulfate solutions. The reaction in 8 M urea is 2nd order, but minor deviations from 2nd order kinetics are observed in sodium dodecyl sulfate. The 2nd order rate constants in 8 M urea are similar to those for the same reaction with cysteine, but the rate of reaction of [beta]-lactoglobulin with N-ethylmaleimide in sodium dodecyl sulfate is about 150 times slower than in 8 M urea. Changes in the spectral properties of the protein occur on reaction with N-ethylmaleimide or HgCl2 under certain conditions of urea concentration and pH. The reaction of ovalbumin with N-ethylmaleimide in sodium dodecyl sulfate does not follow simple 2nd-order kinetics.