ClPLD5, a phospholipase gene is involved in protection of cytoskeleton stability in pollen of self-imcompatible 'Xiangshui' lemon (Citrus limon)
ClPLD5, a phospholipase gene is involved in protection of cytoskeleton stability in pollen of self-imcompatible 'Xiangshui' lemon (Citrus limon)
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DOI：
10.1007/s11240-020-01896-1
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发表时间：
2020-07-26
影响因子：
3
通讯作者：
Hu, Guibing
中科院分区：
文献类型：
--
作者：
Lin, Wei;Minhphu Do;Hu, Guibing
在“响水”柠檬C2-PLD家族中发现了与自交不亲和性相关的关键信息基因AC 2-PLD 5（ClPLD 5），ClPLD 5基因在花粉中的过表达增加了花粉细胞骨架在自交不亲和性挑战下的稳定性，自交不亲和性（SI）是防止植物自交受精的重要机制，也是柑橘无核的主要原因之一。磷脂酶D（PLD）与花粉的生长发育密切相关，但PLD在柑橘花粉SI过程中的作用尚不清楚。在这项研究中，我们鉴定了柑橘植物中与SI相关的PLD，分析了甜橙子和小柑橘基因组中编码C2结构域同源物的15个C2-PLD。通过同源基因分析，对其他物种的PLD亚家族进行了分类，发现香水柠檬的C2-PLD与梨和拟南芥的C2-PLD具有很高的同源性。表达分析表明，ClPLD 5可能参与香水柠檬花粉SI过程的调控。ClPLD 5在花粉中富集，并作为花粉保护因子发挥作用，因为ClPLD 5在自交不亲和花粉中的过表达阻止了SI期间肌动蛋白细胞骨架的解聚。这项工作提供了见解的调节下，柑橘植物花粉的细胞骨架SI挑战，其中PLD起着保护作用，在不兼容的花粉。
Key message AC2-PLDgene (ClPLD5) related to self-incompatibility was identified within the "Xiangshui" lemonC2-PLDfamily, and overexpression of theClPLD5gene in pollen increased the stability of the pollen cytoskeleton under self-incompatibility challenge.Self-incompatibility (SI) is an important mechanism for the prevention of self-fertilization in plants and is one of the major causes of seedlessness in citrus plants. Phospholipase D (PLD) is closely related to the growth and development of pollen, but the role of PLD in citrus plants during SI is poorly understood. In this study, we identified PLDs associated with SI in citrus plants, analyzed 15 C2-PLDs encoding homologs of the C2 domain in the genomes of sweet orange (Citrus sinensis) and clementine mandarin (Citrus clementina). We performed a phylogenic analysis to classify the PLD subfamilies of homologous genes from other species and found that the C2-PLDs of 'Xiangshui' lemon shared high homology with C2-PLDs from pear and Arabidopsis. Expression analysis revealed that ClPLD5 is probably involved in the regulation of pollen of 'Xiangshui' lemon during SI. ClPLD5 is enriched in pollen and functions as a pollen protection factor, as overexpression of ClPLD5 in self-incompatible pollen prevented the depolymerization of the actin cytoskeleton during SI. This work provides insights into the regulation of the cytoskeleton in pollen under SI challenge in citrus plants, in which PLD plays a protective role in incompatible pollen.